In vitro release of [3H]5-hydroxytryptamine from fetal and maternal brain by drugs of abuse

Kenneth Kramer; Efrain C. Azmitia; Patricia M. Whitaker-Azmitia

doi:10.1016/0165-3806(94)90019-1

In vitro release of [³H]5-hydroxytryptamine from fetal and maternal brain by drugs of abuse

Kenneth Kramer, Efrain C. Azmitia, Patricia M. Whitaker-Azmitia

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Cortical synaptosomes were prepared from pregnant dams (GD-17) and rat fetuses (ED-17), loaded with [³H]5-HT and assayed to evaluate release mediated by cocaine (COC), fenfluramine (FEN) and 3,4-methylenedioxymethamphetamine (MDMA). COC and FEN elicited a high-affinity (10^-9 M) release response in fetal tissue which was not apparent in the dam. MDMA-induced release was similar in magnitude in both tissue types. Consequently, the release of 5-HT from developing neurons may be one mechanism by which COC and FEN elicit their teratogenetic effects in utero.

Original language	English (US)
Pages (from-to)	142-146
Number of pages	5
Journal	Developmental Brain Research
Volume	78
Issue number	1
DOIs	https://doi.org/10.1016/0165-3806(94)90019-1
State	Published - Mar 18 1994

Keywords

3,4-Methylenedioxymethamphetamine
Cocaine
Fenfluramine
Inhibition
Synaptosome
Transporter

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

Access to Document

10.1016/0165-3806(94)90019-1

Cite this

@article{5933f09f8eb742378be3fce763bf2b21,

title = "In vitro release of [3H]5-hydroxytryptamine from fetal and maternal brain by drugs of abuse",

abstract = "Cortical synaptosomes were prepared from pregnant dams (GD-17) and rat fetuses (ED-17), loaded with [3H]5-HT and assayed to evaluate release mediated by cocaine (COC), fenfluramine (FEN) and 3,4-methylenedioxymethamphetamine (MDMA). COC and FEN elicited a high-affinity (10-9 M) release response in fetal tissue which was not apparent in the dam. MDMA-induced release was similar in magnitude in both tissue types. Consequently, the release of 5-HT from developing neurons may be one mechanism by which COC and FEN elicit their teratogenetic effects in utero.",

keywords = "3,4-Methylenedioxymethamphetamine, Cocaine, Fenfluramine, Inhibition, Synaptosome, Transporter",

author = "Kenneth Kramer and Azmitia, {Efrain C.} and Whitaker-Azmitia, {Patricia M.}",

note = "Funding Information: Acknowledgements. The authors wish to thank Lauren Kramer and James Bell III for their help in the preparation of this manuscript. This work was supported by NICHD to P.M.W.-A. and by NIDA contract No. 271-90-7403 to E.C.A.",

year = "1994",

month = mar,

day = "18",

doi = "10.1016/0165-3806(94)90019-1",

language = "English (US)",

volume = "78",

pages = "142--146",

journal = "Developmental Brain Research",

issn = "0165-3806",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - In vitro release of [3H]5-hydroxytryptamine from fetal and maternal brain by drugs of abuse

AU - Kramer, Kenneth

AU - Azmitia, Efrain C.

AU - Whitaker-Azmitia, Patricia M.

N1 - Funding Information: Acknowledgements. The authors wish to thank Lauren Kramer and James Bell III for their help in the preparation of this manuscript. This work was supported by NICHD to P.M.W.-A. and by NIDA contract No. 271-90-7403 to E.C.A.

PY - 1994/3/18

Y1 - 1994/3/18

N2 - Cortical synaptosomes were prepared from pregnant dams (GD-17) and rat fetuses (ED-17), loaded with [3H]5-HT and assayed to evaluate release mediated by cocaine (COC), fenfluramine (FEN) and 3,4-methylenedioxymethamphetamine (MDMA). COC and FEN elicited a high-affinity (10-9 M) release response in fetal tissue which was not apparent in the dam. MDMA-induced release was similar in magnitude in both tissue types. Consequently, the release of 5-HT from developing neurons may be one mechanism by which COC and FEN elicit their teratogenetic effects in utero.

AB - Cortical synaptosomes were prepared from pregnant dams (GD-17) and rat fetuses (ED-17), loaded with [3H]5-HT and assayed to evaluate release mediated by cocaine (COC), fenfluramine (FEN) and 3,4-methylenedioxymethamphetamine (MDMA). COC and FEN elicited a high-affinity (10-9 M) release response in fetal tissue which was not apparent in the dam. MDMA-induced release was similar in magnitude in both tissue types. Consequently, the release of 5-HT from developing neurons may be one mechanism by which COC and FEN elicit their teratogenetic effects in utero.

KW - 3,4-Methylenedioxymethamphetamine

KW - Cocaine

KW - Fenfluramine

KW - Inhibition

KW - Synaptosome

KW - Transporter

UR - http://www.scopus.com/inward/record.url?scp=0028175520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028175520&partnerID=8YFLogxK

U2 - 10.1016/0165-3806(94)90019-1

DO - 10.1016/0165-3806(94)90019-1

M3 - Article

C2 - 7911745

AN - SCOPUS:0028175520

SN - 0165-3806

VL - 78

SP - 142

EP - 146

JO - Developmental Brain Research

JF - Developmental Brain Research

IS - 1

ER -