In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography

Bo Wang; Jessica E. Nevins; Zach Nadler; Gadi Wollstein; Hiroshi Ishikawa; Richard A. Bilonick; Larry Kagemann; Ian A. Sigal; Ireneusz Grulkowski; Jonathan J. Liu; Martin Kraus; Chen D. Lu; Joachim Hornegger; James G. Fujimoto; Joel S. Schuman

doi:10.1167/iovs.13-13109

In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography

Bo Wang, Jessica E. Nevins, Zach Nadler, Gadi Wollstein, Hiroshi Ishikawa, Richard A. Bilonick, Larry Kagemann, Ian A. Sigal, Ireneusz Grulkowski, Jonathan J. Liu, Martin Kraus, Chen D. Lu, Joachim Hornegger, James G. Fujimoto, Joel S. Schuman

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

PURPOSE: The lamina cribrosa (LC) is a prime location of glaucomatous damage. The purpose of this study was to compare LC 3-dimensional micro-architecture between healthy and glaucomatous eyes in vivo by using optical coherence tomography (OCT).

METHODS: Sixty-eight eyes (19 healthy and 49 glaucomatous) from 47 subjects were scanned in a 3.5 × 3.5 × 3.64-mm volume (400 × 400 × 896 pixels) at the optic nerve head by using swept-source OCT. The LC micro-architecture parameters were measured on the visible LC by an automated segmentation algorithm. The LC parameters were compared to diagnosis and visual field mean deviation (VF MD) by using a linear mixed effects model accounting for age.

RESULTS: The average VF MD for the healthy and glaucomatous eyes was -0.50 ± 0.80 dB and -7.84 ± 8.75 dB, respectively. Beam thickness to pore diameter ratio (P = 0.04) and pore diameter standard deviation (P < 0.01) were increased in glaucomatous eyes. With worse MD, beam thickness to pore diameter ratio (P < 0.01), pore diameter standard deviation (P = 0.05), and beam thickness (P < 0.01) showed a statistically significant increase while pore diameter (P = 0.02) showed a significant decrease. There were no significant interactions between any of the parameters and age (all P > 0.05).

CONCLUSIONS: Glaucomatous micro-architecture changes in the LC, detected by OCT analysis, reflect beams remodeling and axonal loss leading to reduction in pore size and increased pore size variability.

Original language	English (US)
Pages (from-to)	8270-8274
Number of pages	5
Journal	Investigative ophthalmology & visual science
Volume	54
Issue number	13
DOIs	https://doi.org/10.1167/iovs.13-13109
State	Published - Dec 1 2013

Keywords

glaucoma
lamina cribrosa
optical coherence tomography

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1167/iovs.13-13109

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Wang, B., Nevins, J. E., Nadler, Z., Wollstein, G., Ishikawa, H., Bilonick, R. A., Kagemann, L., Sigal, I. A., Grulkowski, I., Liu, J. J., Kraus, M., Lu, C. D., Hornegger, J., Fujimoto, J. G., & Schuman, J. S. (2013). In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography. Investigative ophthalmology & visual science, 54(13), 8270-8274. https://doi.org/10.1167/iovs.13-13109

Wang, B, Nevins, JE, Nadler, Z, Wollstein, G, Ishikawa, H, Bilonick, RA, Kagemann, L, Sigal, IA, Grulkowski, I, Liu, JJ, Kraus, M, Lu, CD, Hornegger, J, Fujimoto, JG & Schuman, JS 2013, 'In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography', Investigative ophthalmology & visual science, vol. 54, no. 13, pp. 8270-8274. https://doi.org/10.1167/iovs.13-13109

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title = "In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography",

abstract = "PURPOSE: The lamina cribrosa (LC) is a prime location of glaucomatous damage. The purpose of this study was to compare LC 3-dimensional micro-architecture between healthy and glaucomatous eyes in vivo by using optical coherence tomography (OCT).METHODS: Sixty-eight eyes (19 healthy and 49 glaucomatous) from 47 subjects were scanned in a 3.5 × 3.5 × 3.64-mm volume (400 × 400 × 896 pixels) at the optic nerve head by using swept-source OCT. The LC micro-architecture parameters were measured on the visible LC by an automated segmentation algorithm. The LC parameters were compared to diagnosis and visual field mean deviation (VF MD) by using a linear mixed effects model accounting for age.RESULTS: The average VF MD for the healthy and glaucomatous eyes was -0.50 ± 0.80 dB and -7.84 ± 8.75 dB, respectively. Beam thickness to pore diameter ratio (P = 0.04) and pore diameter standard deviation (P < 0.01) were increased in glaucomatous eyes. With worse MD, beam thickness to pore diameter ratio (P < 0.01), pore diameter standard deviation (P = 0.05), and beam thickness (P < 0.01) showed a statistically significant increase while pore diameter (P = 0.02) showed a significant decrease. There were no significant interactions between any of the parameters and age (all P > 0.05).CONCLUSIONS: Glaucomatous micro-architecture changes in the LC, detected by OCT analysis, reflect beams remodeling and axonal loss leading to reduction in pore size and increased pore size variability.",

keywords = "glaucoma, lamina cribrosa, optical coherence tomography",

author = "Bo Wang and Nevins, {Jessica E.} and Zach Nadler and Gadi Wollstein and Hiroshi Ishikawa and Bilonick, {Richard A.} and Larry Kagemann and Sigal, {Ian A.} and Ireneusz Grulkowski and Liu, {Jonathan J.} and Martin Kraus and Lu, {Chen D.} and Joachim Hornegger and Fujimoto, {James G.} and Schuman, {Joel S.}",

year = "2013",

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doi = "10.1167/iovs.13-13109",

language = "English (US)",

volume = "54",

pages = "8270--8274",

journal = "Investigative ophthalmology & visual science",

issn = "0146-0404",

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T1 - In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography

AU - Wang, Bo

AU - Nevins, Jessica E.

AU - Nadler, Zach

AU - Wollstein, Gadi

AU - Ishikawa, Hiroshi

AU - Bilonick, Richard A.

AU - Kagemann, Larry

AU - Sigal, Ian A.

AU - Grulkowski, Ireneusz

AU - Liu, Jonathan J.

AU - Kraus, Martin

AU - Lu, Chen D.

AU - Hornegger, Joachim

AU - Fujimoto, James G.

AU - Schuman, Joel S.

PY - 2013/12/1

Y1 - 2013/12/1

N2 - PURPOSE: The lamina cribrosa (LC) is a prime location of glaucomatous damage. The purpose of this study was to compare LC 3-dimensional micro-architecture between healthy and glaucomatous eyes in vivo by using optical coherence tomography (OCT).METHODS: Sixty-eight eyes (19 healthy and 49 glaucomatous) from 47 subjects were scanned in a 3.5 × 3.5 × 3.64-mm volume (400 × 400 × 896 pixels) at the optic nerve head by using swept-source OCT. The LC micro-architecture parameters were measured on the visible LC by an automated segmentation algorithm. The LC parameters were compared to diagnosis and visual field mean deviation (VF MD) by using a linear mixed effects model accounting for age.RESULTS: The average VF MD for the healthy and glaucomatous eyes was -0.50 ± 0.80 dB and -7.84 ± 8.75 dB, respectively. Beam thickness to pore diameter ratio (P = 0.04) and pore diameter standard deviation (P < 0.01) were increased in glaucomatous eyes. With worse MD, beam thickness to pore diameter ratio (P < 0.01), pore diameter standard deviation (P = 0.05), and beam thickness (P < 0.01) showed a statistically significant increase while pore diameter (P = 0.02) showed a significant decrease. There were no significant interactions between any of the parameters and age (all P > 0.05).CONCLUSIONS: Glaucomatous micro-architecture changes in the LC, detected by OCT analysis, reflect beams remodeling and axonal loss leading to reduction in pore size and increased pore size variability.

AB - PURPOSE: The lamina cribrosa (LC) is a prime location of glaucomatous damage. The purpose of this study was to compare LC 3-dimensional micro-architecture between healthy and glaucomatous eyes in vivo by using optical coherence tomography (OCT).METHODS: Sixty-eight eyes (19 healthy and 49 glaucomatous) from 47 subjects were scanned in a 3.5 × 3.5 × 3.64-mm volume (400 × 400 × 896 pixels) at the optic nerve head by using swept-source OCT. The LC micro-architecture parameters were measured on the visible LC by an automated segmentation algorithm. The LC parameters were compared to diagnosis and visual field mean deviation (VF MD) by using a linear mixed effects model accounting for age.RESULTS: The average VF MD for the healthy and glaucomatous eyes was -0.50 ± 0.80 dB and -7.84 ± 8.75 dB, respectively. Beam thickness to pore diameter ratio (P = 0.04) and pore diameter standard deviation (P < 0.01) were increased in glaucomatous eyes. With worse MD, beam thickness to pore diameter ratio (P < 0.01), pore diameter standard deviation (P = 0.05), and beam thickness (P < 0.01) showed a statistically significant increase while pore diameter (P = 0.02) showed a significant decrease. There were no significant interactions between any of the parameters and age (all P > 0.05).CONCLUSIONS: Glaucomatous micro-architecture changes in the LC, detected by OCT analysis, reflect beams remodeling and axonal loss leading to reduction in pore size and increased pore size variability.

KW - glaucoma

KW - lamina cribrosa

KW - optical coherence tomography

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In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography

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