TY - JOUR
T1 - In vivo optical tissue differentiation by diffuse reflectance spectroscopy
T2 - Preliminary results for tissue-specific laser surgery
AU - Stelzle, Florian
AU - Adler, Werner
AU - Zam, Azhar
AU - Tangermann-Gerk, Katja
AU - Knipfer, Christian
AU - Douplik, Alexandre
AU - Schmidt, Michael
AU - Nkenke, Emeka
PY - 2012/12
Y1 - 2012/12
N2 - Objectives. Laser surgery requires feedback to avoid the accidental destruction of critically important tissues. It was the aim of the authors to identify different tissue types in vivo by diffuse reflectance spectroscopy to set the basis for tissue-specific control of laser surgery. Methods. Tissue differentiation was performed on in vivo tissue of rats (skin, fat, muscle, and nerve) by diffuse reflectance spectroscopy between 350 and 650 nm. Data analysis was done using principal components analysis, followed by linear discriminant analysis (LDA). The differentiation performance was evaluated by receiver operating characteristic (ROC) analysis. Results. ROC analysis showed a tissue differentiation of 100%, with a high sensitivity of more than 99%. Only the tissue pair skin/fat showed a reduced differentiation performance and specificity. Conclusion. The results show the general viability of in vivo optical tissue differentiation and create a basis for the further development of a control system for tissue-specific laser surgery.
AB - Objectives. Laser surgery requires feedback to avoid the accidental destruction of critically important tissues. It was the aim of the authors to identify different tissue types in vivo by diffuse reflectance spectroscopy to set the basis for tissue-specific control of laser surgery. Methods. Tissue differentiation was performed on in vivo tissue of rats (skin, fat, muscle, and nerve) by diffuse reflectance spectroscopy between 350 and 650 nm. Data analysis was done using principal components analysis, followed by linear discriminant analysis (LDA). The differentiation performance was evaluated by receiver operating characteristic (ROC) analysis. Results. ROC analysis showed a tissue differentiation of 100%, with a high sensitivity of more than 99%. Only the tissue pair skin/fat showed a reduced differentiation performance and specificity. Conclusion. The results show the general viability of in vivo optical tissue differentiation and create a basis for the further development of a control system for tissue-specific laser surgery.
KW - diffuse reflectance
KW - optical tissue differentiation
KW - principal components analysis
KW - remote optical measurement
KW - remote surgical methods
KW - spectra analysis
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U2 - 10.1177/1553350611429692
DO - 10.1177/1553350611429692
M3 - Article
C2 - 22344924
AN - SCOPUS:84868539717
SN - 1553-3506
VL - 19
SP - 385
EP - 393
JO - Surgical Innovation
JF - Surgical Innovation
IS - 4
ER -