TY - JOUR
T1 - Incorporation of Macromolecules into α-Lactose Monohydrate Crystals
AU - Shtukenberg, Alexander G.
AU - Tripathi, Kelly
AU - Ketchum, Remi
AU - Jeon, Jenny Jaehee
AU - Sanda, Ahmed
AU - Kahr, Bart
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/8/3
Y1 - 2016/8/3
N2 - It had been discovered that crystals of α-lactose monohydrate can occlude and give kinetic stability to various proteins, an unexpected observation suggesting a process for storing protein vaccines and biopharmaceuticals at room temperature. The growth of α-lactose monohydrate with proteins is analyzed herein, emphasizing segregation coefficients, the relationships between protein incorporation and buffer composition, temperature, growth rate, and mass transport. Detailed examination of bovine serum albumin is supported by data for eight other proteins. A search for other crystals with lactose-like inclusivity of macromolecular guests is also reported.
AB - It had been discovered that crystals of α-lactose monohydrate can occlude and give kinetic stability to various proteins, an unexpected observation suggesting a process for storing protein vaccines and biopharmaceuticals at room temperature. The growth of α-lactose monohydrate with proteins is analyzed herein, emphasizing segregation coefficients, the relationships between protein incorporation and buffer composition, temperature, growth rate, and mass transport. Detailed examination of bovine serum albumin is supported by data for eight other proteins. A search for other crystals with lactose-like inclusivity of macromolecular guests is also reported.
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U2 - 10.1021/acs.cgd.6b00686
DO - 10.1021/acs.cgd.6b00686
M3 - Article
AN - SCOPUS:84982719890
SN - 1528-7483
VL - 16
SP - 4589
EP - 4598
JO - Crystal Growth and Design
JF - Crystal Growth and Design
IS - 8
ER -