Increased length of long terminal repeats inhibits Ty1 transposition and leads to the formation of tandem multimers

Vit Lauermann, Monika Hermankova, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    The Ty1 retrotransposon of Saccharomyces cerevisiae is bounded by long- terminal repeats (LTRs). We have constructed a variety of Ty1 elements in which the LTR length has been increased from the normal length of 334 bp to >2 kb. Although small insertions in the LTR have minimal effects on transposition frequency, larger insertions dramatically reduce it. Nevertheless, elements with long LTRs are incorporated into the genome at a low frequency. Most of these rare insertion events represent Ty1 tandem (head to tail) multimers.

    Original languageEnglish (US)
    Pages (from-to)911-922
    Number of pages12
    JournalGenetics
    Volume145
    Issue number4
    StatePublished - Apr 1997

    ASJC Scopus subject areas

    • Genetics

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