Abstract
In this study, we demonstrate that increasing the surface conductance of the ion-selective membrane can achieve higher Ion concentration polarization (ICP) of DNA. The surface conductance of the conductive polymer membrane, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), can be enhanced either by increasing its thickness or surface charge. Using a ∼6 μm thick PEDOT:PSS membrane with a highly negative surface charge, we could achieve a concentration increase of DNA by 6 orders of magnitude from an initial concentration of 100 fM within 10 min., and detect a DNA target concentration at 10 pM within 15 min. via surface hybridization on the morpholinos (MO).
| Original language | English (US) |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 896-897 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| State | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: Oct 9 2016 → Oct 13 2016 |
Publication series
| Name | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country | Ireland |
| City | Dublin |
| Period | 10/9/16 → 10/13/16 |
Keywords
- DNA hybridization
- Ion concentration polarization
- Morpholinos (MO)
- PEDOT:PSS
ASJC Scopus subject areas
- Control and Systems Engineering
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Cite this
Wei, X., Zhang, Q., & Song, Y. A. (2016). Increasing the detection sensitivity for dnamorpholino hybridization in sub-nanomolar regime by enhancing the surface ion conductance of PEDOT: PSS membrane in a microchannel. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 896-897). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.