TY - GEN
T1 - Increasing the detection sensitivity for dnamorpholino hybridization in sub-nanomolar regime by enhancing the surface ion conductance of PEDOT
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
AU - Wei, X.
AU - Zhang, Q.
AU - Song, Y. A.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - In this study, we demonstrate that increasing the surface conductance of the ion-selective membrane can achieve higher Ion concentration polarization (ICP) of DNA. The surface conductance of the conductive polymer membrane, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), can be enhanced either by increasing its thickness or surface charge. Using a ∼6 μm thick PEDOT:PSS membrane with a highly negative surface charge, we could achieve a concentration increase of DNA by 6 orders of magnitude from an initial concentration of 100 fM within 10 min., and detect a DNA target concentration at 10 pM within 15 min. via surface hybridization on the morpholinos (MO).
AB - In this study, we demonstrate that increasing the surface conductance of the ion-selective membrane can achieve higher Ion concentration polarization (ICP) of DNA. The surface conductance of the conductive polymer membrane, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), can be enhanced either by increasing its thickness or surface charge. Using a ∼6 μm thick PEDOT:PSS membrane with a highly negative surface charge, we could achieve a concentration increase of DNA by 6 orders of magnitude from an initial concentration of 100 fM within 10 min., and detect a DNA target concentration at 10 pM within 15 min. via surface hybridization on the morpholinos (MO).
KW - DNA hybridization
KW - Ion concentration polarization
KW - Morpholinos (MO)
KW - PEDOT:PSS
UR - http://www.scopus.com/inward/record.url?scp=85014303134&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014303134&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014303134
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 896
EP - 897
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
Y2 - 9 October 2016 through 13 October 2016
ER -