Increasing the detection sensitivity for dnamorpholino hybridization in sub-nanomolar regime by enhancing the surface ion conductance of PEDOT: PSS membrane in a microchannel

X. Wei, Q. Zhang, Y. A. Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we demonstrate that increasing the surface conductance of the ion-selective membrane can achieve higher Ion concentration polarization (ICP) of DNA. The surface conductance of the conductive polymer membrane, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), can be enhanced either by increasing its thickness or surface charge. Using a ∼6 μm thick PEDOT:PSS membrane with a highly negative surface charge, we could achieve a concentration increase of DNA by 6 orders of magnitude from an initial concentration of 100 fM within 10 min., and detect a DNA target concentration at 10 pM within 15 min. via surface hybridization on the morpholinos (MO).

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages896-897
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • DNA hybridization
  • Ion concentration polarization
  • Morpholinos (MO)
  • PEDOT:PSS

ASJC Scopus subject areas

  • Control and Systems Engineering

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