Initial analytic quality assessment and method comparison of an immunoassay for adrenocorticotropic hormone measurement in equine samples

KL Irvine, Kay Burt, Andrew J Hill, Stephanie Shaw, Kostas Papasouliotis, Nidal Hilal

Research output: Contribution to journalArticlepeer-review

Abstract

Background
Equine pituitary pars intermedia dysfunction (PPID) may be diagnosed by measuring baseline plasma adrenocorticotrophic hormone (ACTH). The Immulite 1000 analyzer uses an automated chemiluminescence enzyme assay, previously validated for measuring equine ACTH. Recently, an automated bench‐top immunoassay analyzer (AIA‐360), designed for analytes in people, became available for veterinary use.
Objectives
Objectives were to evaluate analytic performance of the AIA immunoassay for measuring equine ACTH, and compare the results with those obtained by the Immulite.
Methods
Adrenocorticotrophic hormone was measured in plasma samples from 52 clinical cases. For the AIA, within‐ and between‐run coefficients of variation (CV) were assessed, linearity and recovery studies performed, and observed total error (TEobs) calculated.
Original languageEnglish (US)
Pages (from-to)154-163
JournalVeterinary Clinical Pathology
Volume45
Issue number1
StatePublished - Mar 2016

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