TY - JOUR
T1 - Insights from Bacterial 16S rRNA Gene into Bacterial Genera and Predicted Metabolic Pathways Associated with Stool Consistency in Rectal Cancer Patients
T2 - A Proof of Concept
AU - Gonzalez-Mercado, Velda Janet
AU - Lim, Jean
AU - Aouizerat, Bradley
N1 - Funding Information:
We thank all the staff at Tampa General Hospital Cancer Center, the Cancer Care team at AdventHealthTampa, and the St Joseph’s Hospital Cancer Institute for the collaborative clinical recruitment support. This study would not have been possible without the participants. We also thank the Biobehavioral Laboratory at University of South Florida’s College of Nursing for Illumina sequencing support. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the National Institute of Nursing Research (NINR) of the National Institutes of Health (NIH) under Award number K23NR020039 and University of Puerto Rico (UPR) NIH grant awards 2U54MD007587 and CA096297/CA096300. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the National Institute of Nursing Research (NINR) of the National Institutes of Health (NIH) under Award number K23NR020039 and University of Puerto Rico (UPR) NIH grant awards 2U54MD007587 and CA096297/CA096300. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© The Author(s) 2023.
PY - 2023/7
Y1 - 2023/7
N2 - Purpose: To examine if gut microbial taxa abundances and predicted functional pathways correlate with Bristol Stool Form Scale (BSFS) classification at the end of neoadjuvant chemotherapy and radiation therapy (CRT) for rectal cancer. Methods: Rectal cancer patients (n = 39) provided stool samples for 16S rRNA gene sequencing. Stool consistency was evaluated using the BSFS. Gut microbiome data were analyzed using QIIME2. Correlation analysis were performed in R. Results: At the genus level, Staphylococcus positively correlates (Spearman’s rho = 0.26), while Anaerofustis, Roseburia, Peptostreptococcaceae unclassified, Ruminococcaceae UBA1819, Shuttleworthia, Ca. Soleaferrea, Anaerostignum, Oscillibacter, and Akkermansia negatively correlate with BSFS scores (Spearman’s rho −0.20 to −0.42). Predicted pathways, including mycothiol biosynthesis and sucrose degradation III (sucrose invertase), were positively correlated with BSFS (Spearman’s rho = 0.03–0.21). Conclusion: The data support that in rectal cancer patients, stool consistency is an important factor to include in microbiome studies. Loose/liquid stools may be linked to Staphylococcus abundance and to mycothiol biosynthesis and sucrose degradation pathways.
AB - Purpose: To examine if gut microbial taxa abundances and predicted functional pathways correlate with Bristol Stool Form Scale (BSFS) classification at the end of neoadjuvant chemotherapy and radiation therapy (CRT) for rectal cancer. Methods: Rectal cancer patients (n = 39) provided stool samples for 16S rRNA gene sequencing. Stool consistency was evaluated using the BSFS. Gut microbiome data were analyzed using QIIME2. Correlation analysis were performed in R. Results: At the genus level, Staphylococcus positively correlates (Spearman’s rho = 0.26), while Anaerofustis, Roseburia, Peptostreptococcaceae unclassified, Ruminococcaceae UBA1819, Shuttleworthia, Ca. Soleaferrea, Anaerostignum, Oscillibacter, and Akkermansia negatively correlate with BSFS scores (Spearman’s rho −0.20 to −0.42). Predicted pathways, including mycothiol biosynthesis and sucrose degradation III (sucrose invertase), were positively correlated with BSFS (Spearman’s rho = 0.03–0.21). Conclusion: The data support that in rectal cancer patients, stool consistency is an important factor to include in microbiome studies. Loose/liquid stools may be linked to Staphylococcus abundance and to mycothiol biosynthesis and sucrose degradation pathways.
KW - biosynthesis/functional pathways
KW - gut microbiome
KW - mechanisms
KW - rectal cancer
KW - stool compositions
KW - stool form
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U2 - 10.1177/10998004231159623
DO - 10.1177/10998004231159623
M3 - Article
C2 - 36859821
AN - SCOPUS:85150359445
SN - 1099-8004
VL - 25
SP - 491
EP - 500
JO - Biological Research for Nursing
JF - Biological Research for Nursing
IS - 3
ER -