Instrumentation for simultaneous magnetic resonance and optical tomographic imaging of the rodent brain

James M. Masciotti, Jonghwan Lee, Mark Stewart, Andreas H. Hielscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We present an instrument for simultaneous imaging of the rodent brain with frequency-domain optical tomography and magnetic resonance imaging. The instrument uses a custom-built fiber optic probe that allows for measurements in backreflectance geometry. The probe consists of 13 source and 26 detector fibers and is small enough to fit inside of a microMRI RF coil with an inner diameter of 38mm. Illumination by the source fibers is time demultiplexed by an optical fiber switch. A gain-modulated image intensifier CCD camera focuses onto the endpoints of large-core gradedindex detector fibers and collects the frequency-domain data. Imaging can be performed with source-modulation frequencies up to 1 GHz. The instrument is capable of acquiring multi-frequency optical tomography data at 2 wavelengths, and the data can be used to generate 3D maps of hemoglobin concentrations. At the same time magnetic resonance images can be acquired with in-plane resolution smaller than 100 micron. To illustrate the performance of the instrument we show results of small animal studies that involve inhalation of 100% carbogen and chemically induced seizures.

Original languageEnglish (US)
Title of host publicationMultimodal Biomedical Imaging IV
StatePublished - 2009
EventMultimodal Biomedical Imaging IV - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


ConferenceMultimodal Biomedical Imaging IV
Country/TerritoryUnited States
CitySan Jose, CA


  • MRI
  • Magnetic resonance imaging
  • Multimodal imaging
  • Multimodality
  • Optical tomography
  • Rat brain

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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