Integrated and diffusion-based micro-injectors for open access cell assays

Xin Li, Li Liu, Li Wang, Ken Ichiro Kamei, Qinghua Yuan, Fan Zhang, Jian Shi, Akihiro Kusumi, Min Xie, Zhenjie Zhao, Yong Chen

Research output: Contribution to journalArticlepeer-review


Currently, most microfluidic devices are fabricated with embedded micro-channels and other elements in a close form with outward connections. Although much functionality has been demonstrated and a large number of applications have been developed, they are not easy for routine operation in biology laboratories where most in vitro cell processing still relies on the use of culture dishes, glass slides, multi-well plates, tubes, pipettes, etc. We report here an open access device which consists of an array of isolated micro-channels plated on a large culture surface, each of them having tiny nozzles for localized drug delivery. In a diffusion dominant regime, steady gradients of molecule concentration could be obtained and varied by changing the flow rate inside the micro-channels. As assay examples, cell staining and drug-induced cell apoptosis were demonstrated, showing fast cell responses in close proximity of the nozzles.

Original languageEnglish (US)
Pages (from-to)2612-2617
Number of pages6
JournalLab on a Chip
Issue number15
StatePublished - Aug 7 2011

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering


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