Integrated microfluidic devices for combinatorial cell-based assays

Zeta Tak For Yu, Ken Ichiro Kamei, Hiroko Takahashi, Chengyi Jenny Shu, Xiaopu Wang, George Wenfu He, Robert Silverman, Caius G. Radu, Owen N. Witte, Ki Bum Lee, Hsian Rong Tseng

Research output: Contribution to journalArticlepeer-review


The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-μChip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-μChip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.

Original languageEnglish (US)
Pages (from-to)547-555
Number of pages9
JournalBiomedical Microdevices
Issue number3
StatePublished - 2009


  • Apoptosis
  • Cell culture
  • Cell-based assay
  • Microfluidic devices
  • Transfection

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology


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