Integrated Microfluidic Probe for Single Cell Manipulation

Samuel Sofela, Alla Saleh, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the study of single cells, maintaining spatiotemporal integrity of cells is crucial for reliable omics analysis. Most techniques utilize suspended cells which disrupts their spatial arrangement in native tissues and introduce measures of compromise in the experimental data. To this end, we have developed an integrated microfluidic probe which combines principles of electropermeabilization and hydrodynamic flow confinement for minimally-invasive biopsy of adherent cells, and potentially tissues slices. We used our probe to extract the cytoplasmic content of adherent PC3 and HeLa cells in a confluent monolayer of culture, and evaluated the expression of a target gene. We further advanced the functionality of the probe by incorporating droplet microfluidics for compartmentalization of retrieved cytoplasmic content from different cells, for potentially improving the experimental throughput of this technology.

Original languageEnglish (US)
Title of host publicationProceedings of MARSS 2022 - 5th International Conference on Manipulation, Automation, and Robotics at Small Scales
EditorsSinan Haliyo, Mokrane Boudaoud, Eric Diller, Xinyu Liu, Yu Sun, Sergej Fatikow
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781665459730
DOIs
StatePublished - 2022
Event5th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2022 - Toronto, Canada
Duration: Jul 25 2022Jul 29 2022

Publication series

NameProceedings of MARSS 2022 - 5th International Conference on Manipulation, Automation, and Robotics at Small Scales

Conference

Conference5th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2022
Country/TerritoryCanada
CityToronto
Period7/25/227/29/22

Keywords

  • 3D printing
  • droplet microfluidics
  • electropermeabilization
  • Integrated microfluidic probe
  • microfluidics
  • single-cell manipulation

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Science Applications
  • Mechanical Engineering
  • Control and Optimization

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