TY - GEN
T1 - Integrated Microfluidic Probe for Single Cell Manipulation
AU - Sofela, Samuel
AU - Saleh, Alla
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - In the study of single cells, maintaining spatiotemporal integrity of cells is crucial for reliable omics analysis. Most techniques utilize suspended cells which disrupts their spatial arrangement in native tissues and introduce measures of compromise in the experimental data. To this end, we have developed an integrated microfluidic probe which combines principles of electropermeabilization and hydrodynamic flow confinement for minimally-invasive biopsy of adherent cells, and potentially tissues slices. We used our probe to extract the cytoplasmic content of adherent PC3 and HeLa cells in a confluent monolayer of culture, and evaluated the expression of a target gene. We further advanced the functionality of the probe by incorporating droplet microfluidics for compartmentalization of retrieved cytoplasmic content from different cells, for potentially improving the experimental throughput of this technology.
AB - In the study of single cells, maintaining spatiotemporal integrity of cells is crucial for reliable omics analysis. Most techniques utilize suspended cells which disrupts their spatial arrangement in native tissues and introduce measures of compromise in the experimental data. To this end, we have developed an integrated microfluidic probe which combines principles of electropermeabilization and hydrodynamic flow confinement for minimally-invasive biopsy of adherent cells, and potentially tissues slices. We used our probe to extract the cytoplasmic content of adherent PC3 and HeLa cells in a confluent monolayer of culture, and evaluated the expression of a target gene. We further advanced the functionality of the probe by incorporating droplet microfluidics for compartmentalization of retrieved cytoplasmic content from different cells, for potentially improving the experimental throughput of this technology.
KW - 3D printing
KW - Integrated microfluidic probe
KW - droplet microfluidics
KW - electropermeabilization
KW - microfluidics
KW - single-cell manipulation
UR - http://www.scopus.com/inward/record.url?scp=85139084858&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85139084858&partnerID=8YFLogxK
U2 - 10.1109/MARSS55884.2022.9870475
DO - 10.1109/MARSS55884.2022.9870475
M3 - Conference contribution
AN - SCOPUS:85139084858
T3 - Proceedings of MARSS 2022 - 5th International Conference on Manipulation, Automation, and Robotics at Small Scales
BT - Proceedings of MARSS 2022 - 5th International Conference on Manipulation, Automation, and Robotics at Small Scales
A2 - Haliyo, Sinan
A2 - Boudaoud, Mokrane
A2 - Diller, Eric
A2 - Liu, Xinyu
A2 - Sun, Yu
A2 - Fatikow, Sergej
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 5th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2022
Y2 - 25 July 2022 through 29 July 2022
ER -