Integrated microfluidic probes for cell manipulation and analysis

Ayoola Brimmo, Ayoub Glia, Pavithra Sukumar, Roaa Alnemari, Anoop Menachery, Muhammedin Deliorman, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The microfluidic probe (MFP) is a non-contact technology that applies the concept of hydrodynamic flow confinement within a small gap to eliminate the need for closed conduits, and thus overcomes the conventional microfluidic "closed system" limitations. It is an open-space microfluidic concept, where the fluidic delivery mechanism is physically decoupled from the target surface to be processed such as tissue slices or cell culture in Petri dishes. Typically, MFPs are manufactured using complex photolithography-based microfabrication procedures that limits innovation in MFPs' design and integration. Recently, we showed that 3D printing can be utilized for rapid microfabrication of MFPs, where MFPs can be manufactured with built-in components such as reservoirs, fluidic connectors, and interfaces to the XYZ probe holder. 3D printing brings flexibility in MFP design, where different configurations and aperture arrangements can be considered. Currently, we are developing advanced MFPs that are integrated with other technologies and targeting applications in dielectrophoretic-based cell separation, immuno-based cell capture for isolating circulating tumor cells from blood samples, and efficient and selective single cell electroporation. In this invited paper, we highlight several MFP technologies we are developing.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems XVII
EditorsBonnie L. Gray, Holger Becker
PublisherSPIE
ISBN (Electronic)9781510623927
DOIs
StatePublished - 2019
EventMicrofluidics, BioMEMS, and Medical Microsystems XVII 2019 - San Francisco, United States
Duration: Feb 2 2019Feb 4 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10875
ISSN (Print)1605-7422

Conference

ConferenceMicrofluidics, BioMEMS, and Medical Microsystems XVII 2019
CountryUnited States
CitySan Francisco
Period2/2/192/4/19

Keywords

  • 3D printing
  • Concentration gradient
  • Dielectrophoresis
  • Electroporation
  • Herringbone micro-mixers
  • Micro electrodes
  • Microfluidic probe
  • Microfluidic quadrupole

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Brimmo, A., Glia, A., Sukumar, P., Alnemari, R., Menachery, A., Deliorman, M., & Qasaimeh, M. A. (2019). Integrated microfluidic probes for cell manipulation and analysis. In B. L. Gray, & H. Becker (Eds.), Microfluidics, BioMEMS, and Medical Microsystems XVII [108750R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10875). SPIE. https://doi.org/10.1117/12.2515270