Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces

W. O. Soboyejo, B. Nemetski, S. Allameh, N. Marcantonio, C. Mercer, J. Ricci

Research output: Contribution to journalArticlepeer-review


This paper presents the results of an experimental study of the interactions between MC3T3-E1 (mouse calvarian) cells and textured Ti6A14V surfaces, including surfaces produced by laser microgrooving; blasting with alumina particles; and polishing. The multiscale interactions between MC3T3-E1 cells and these textured surfaces are studied using a combination of optical scanning transmission electron microscopy and atomic force microscopy. The potential cytotoxic effects of microchemistry on cell-surface interactions also are considered in studies of cell spreading and orientation over 9-day periods. These studies show that cells on microgrooved Ti6A14V geometries that are 8 or 12 μm deep undergo contact guidance and limited cell spreading. Similar contact guidance is observed on the surfaces of diamond-polished surfaces on which nanoscale grooves are formed due to the scratching that occurs during polishing. In contrast, random cell orientations are observed on alumina-blasted Ti6A14V surfaces. The possible effects of surface topography are discussed for scar-tissue formation and improved cell-surface integration.

Original languageEnglish (US)
Pages (from-to)56-72
Number of pages17
JournalJournal of Biomedical Materials Research
Issue number1
StatePublished - 2002


  • Cell-surface interactions
  • Laser microgrooves
  • MC3T3-E1 cells
  • Surface textures
  • Ti6Al4V

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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