Abstract
This paper presents the results of an experimental study of the interactions between MC3T3-E1 (mouse calvarian) cells and textured Ti6A14V surfaces, including surfaces produced by laser microgrooving; blasting with alumina particles; and polishing. The multiscale interactions between MC3T3-E1 cells and these textured surfaces are studied using a combination of optical scanning transmission electron microscopy and atomic force microscopy. The potential cytotoxic effects of microchemistry on cell-surface interactions also are considered in studies of cell spreading and orientation over 9-day periods. These studies show that cells on microgrooved Ti6A14V geometries that are 8 or 12 μm deep undergo contact guidance and limited cell spreading. Similar contact guidance is observed on the surfaces of diamond-polished surfaces on which nanoscale grooves are formed due to the scratching that occurs during polishing. In contrast, random cell orientations are observed on alumina-blasted Ti6A14V surfaces. The possible effects of surface topography are discussed for scar-tissue formation and improved cell-surface integration.
Original language | English (US) |
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Pages (from-to) | 56-72 |
Number of pages | 17 |
Journal | Journal of Biomedical Materials Research |
Volume | 62 |
Issue number | 1 |
DOIs | |
State | Published - 2002 |
Keywords
- Cell-surface interactions
- Laser microgrooves
- MC3T3-E1 cells
- Surface textures
- Ti6Al4V
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering