Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides

V. Ibanez, N. E. Geacintov, A. Slama-Schwok, M. Rougée, T. Montenay-Garestier, J. M. Lehn, C. Héléne

Research output: Contribution to journalArticle

Abstract

The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.

Original languageEnglish (US)
Pages (from-to)3234-3242
Number of pages9
JournalBiochemistry
Volume28
Issue number8
DOIs
StatePublished - Apr 1989

ASJC Scopus subject areas

  • Biochemistry

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    Ibanez, V., Geacintov, N. E., Slama-Schwok, A., Rougée, M., Montenay-Garestier, T., Lehn, J. M., & Héléne, C. (1989). Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides. Biochemistry, 28(8), 3234-3242. https://doi.org/10.1021/bi00434a018