Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides

V. Ibanez; N. E. Geacintov; A. Slama-Schwok; M. Rougée; T. Montenay-Garestier; J. M. Lehn; C. Héléne

doi:10.1021/bi00434a018

Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides

V. Ibanez, N. E. Geacintov, A. Slama-Schwok, M. Rougée, T. Montenay-Garestier, J. M. Lehn, C. Héléne

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.

Original language	English (US)
Pages (from-to)	3234-3242
Number of pages	9
Journal	Biochemistry
Volume	28
Issue number	8
DOIs	https://doi.org/10.1021/bi00434a018
State	Published - Apr 1989

ASJC Scopus subject areas

Biochemistry

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abstract = "The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.",

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year = "1989",

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doi = "10.1021/bi00434a018",

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AU - Ibanez, V.

AU - Geacintov, N. E.

AU - Slama-Schwok, A.

AU - Rougée, M.

AU - Montenay-Garestier, T.

AU - Lehn, J. M.

AU - Héléne, C.

PY - 1989/4

Y1 - 1989/4

N2 - The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.

AB - The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.

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Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides

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