Interdependence of CD3-Ti and CD2 activation pathways in human T lymphocytes.

A. Alcover; C. Alberini; O. Acuto; L. K. Clayton; C. Transy; G. C. Spagnoli; P. Moingeon; P. Lopez; E. L. Reinherz

doi:10.1002/j.1460-2075.1988.tb03035.x

Interdependence of CD3-Ti and CD2 activation pathways in human T lymphocytes.

A. Alcover, C. Alberini, O. Acuto, L. K. Clayton, C. Transy, G. C. Spagnoli, P. Moingeon, P. Lopez, E. L. Reinherz

Neural Science

Research output: Contribution to journal › Article › peer-review

Abstract

Human T lymphocytes can be activated through either the antigen/MHC receptor complex T3-Ti (CD3-Ti) or the T11 (CD2) molecule to proliferate via an IL-2 dependent mechanism. To investigate the relationship of these pathways to one another, we generated and characterized Jurkat mutants which selectively express either surface CD3-Ti or CD2. Here we show that CD3-Ti- mutants fail to be stimulated by either pathway to increase phosphoinositide turnover, mobilize calcium or induce the IL-2 gene. The activation capacity of these mutants via CD2 as well as CD3-Ti can be restored following reconstitution of surface CD3-Ti expression upon appropriate DNA transfer (e.g. Ti beta subunit cDNA into Ti beta- Jurkat variants). Collectively, these results demonstrate that CD3-Ti and CD2 pathways are interdependent and that phosphoinositide turnover is linked to the CD3-Ti complex.

Original language	English (US)
Pages (from-to)	1973-1977
Number of pages	5
Journal	The EMBO journal
Volume	7
Issue number	7
DOIs	https://doi.org/10.1002/j.1460-2075.1988.tb03035.x
State	Published - Jul 1988

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1002/j.1460-2075.1988.tb03035.x

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title = "Interdependence of CD3-Ti and CD2 activation pathways in human T lymphocytes.",

abstract = "Human T lymphocytes can be activated through either the antigen/MHC receptor complex T3-Ti (CD3-Ti) or the T11 (CD2) molecule to proliferate via an IL-2 dependent mechanism. To investigate the relationship of these pathways to one another, we generated and characterized Jurkat mutants which selectively express either surface CD3-Ti or CD2. Here we show that CD3-Ti- mutants fail to be stimulated by either pathway to increase phosphoinositide turnover, mobilize calcium or induce the IL-2 gene. The activation capacity of these mutants via CD2 as well as CD3-Ti can be restored following reconstitution of surface CD3-Ti expression upon appropriate DNA transfer (e.g. Ti beta subunit cDNA into Ti beta- Jurkat variants). Collectively, these results demonstrate that CD3-Ti and CD2 pathways are interdependent and that phosphoinositide turnover is linked to the CD3-Ti complex.",

author = "A. Alcover and C. Alberini and O. Acuto and Clayton, {L. K.} and C. Transy and Spagnoli, {G. C.} and P. Moingeon and P. Lopez and Reinherz, {E. L.}",

year = "1988",

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doi = "10.1002/j.1460-2075.1988.tb03035.x",

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T1 - Interdependence of CD3-Ti and CD2 activation pathways in human T lymphocytes.

AU - Alcover, A.

AU - Alberini, C.

AU - Acuto, O.

AU - Clayton, L. K.

AU - Transy, C.

AU - Spagnoli, G. C.

AU - Moingeon, P.

AU - Lopez, P.

AU - Reinherz, E. L.

PY - 1988/7

Y1 - 1988/7

N2 - Human T lymphocytes can be activated through either the antigen/MHC receptor complex T3-Ti (CD3-Ti) or the T11 (CD2) molecule to proliferate via an IL-2 dependent mechanism. To investigate the relationship of these pathways to one another, we generated and characterized Jurkat mutants which selectively express either surface CD3-Ti or CD2. Here we show that CD3-Ti- mutants fail to be stimulated by either pathway to increase phosphoinositide turnover, mobilize calcium or induce the IL-2 gene. The activation capacity of these mutants via CD2 as well as CD3-Ti can be restored following reconstitution of surface CD3-Ti expression upon appropriate DNA transfer (e.g. Ti beta subunit cDNA into Ti beta- Jurkat variants). Collectively, these results demonstrate that CD3-Ti and CD2 pathways are interdependent and that phosphoinositide turnover is linked to the CD3-Ti complex.

AB - Human T lymphocytes can be activated through either the antigen/MHC receptor complex T3-Ti (CD3-Ti) or the T11 (CD2) molecule to proliferate via an IL-2 dependent mechanism. To investigate the relationship of these pathways to one another, we generated and characterized Jurkat mutants which selectively express either surface CD3-Ti or CD2. Here we show that CD3-Ti- mutants fail to be stimulated by either pathway to increase phosphoinositide turnover, mobilize calcium or induce the IL-2 gene. The activation capacity of these mutants via CD2 as well as CD3-Ti can be restored following reconstitution of surface CD3-Ti expression upon appropriate DNA transfer (e.g. Ti beta subunit cDNA into Ti beta- Jurkat variants). Collectively, these results demonstrate that CD3-Ti and CD2 pathways are interdependent and that phosphoinositide turnover is linked to the CD3-Ti complex.

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JO - The EMBO journal

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Interdependence of CD3-Ti and CD2 activation pathways in human T lymphocytes.

Abstract

ASJC Scopus subject areas

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