Interdependence of CD3-Ti and CD2 activation pathways in human T lymphocytes.

A. Alcover, C. Alberini, O. Acuto, L. K. Clayton, C. Transy, G. C. Spagnoli, P. Moingeon, P. Lopez, E. L. Reinherz

Research output: Contribution to journalArticlepeer-review

Abstract

Human T lymphocytes can be activated through either the antigen/MHC receptor complex T3-Ti (CD3-Ti) or the T11 (CD2) molecule to proliferate via an IL-2 dependent mechanism. To investigate the relationship of these pathways to one another, we generated and characterized Jurkat mutants which selectively express either surface CD3-Ti or CD2. Here we show that CD3-Ti- mutants fail to be stimulated by either pathway to increase phosphoinositide turnover, mobilize calcium or induce the IL-2 gene. The activation capacity of these mutants via CD2 as well as CD3-Ti can be restored following reconstitution of surface CD3-Ti expression upon appropriate DNA transfer (e.g. Ti beta subunit cDNA into Ti beta- Jurkat variants). Collectively, these results demonstrate that CD3-Ti and CD2 pathways are interdependent and that phosphoinositide turnover is linked to the CD3-Ti complex.

Original languageEnglish (US)
Pages (from-to)1973-1977
Number of pages5
JournalThe EMBO journal
Volume7
Issue number7
DOIs
StatePublished - Jul 1988

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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