Type I NOS expression increases in OB neurons during VSV infection. Immunocytochemical Staining of NB41A3 cells indicates constitutive expression of interferon (IFN)-γ receptor and type I NOS. IFN-γ treatment of NB41A3 cells increased NO production and type 1 NOS protein. In vitro replication of VSV, polio virus type 1, and Herpes Simplex virus type 1 (HSV-1) is significantly inhibited by IFN-γ induced type I NOS and antagonized by NOS inhibitors. In contrast, while IFN-γ treatment inhibited influenza and Sindbis virus replication, a different pathway(s) was involved. The isoform-selective NOS inhibitor, 7-nitroindazole (7NI) was used to treat mice, resulting in a 10-fold higher titer of virus in brain homogenates, and abrogated the recovery-promoting effect of interleukin-12 treatment. Thus, IFN-γ induced type I NOS activity may play an important role in host immunity against neurotropic viral infections.
- Neuronal nitric oxide synthase
- Viral infection
ASJC Scopus subject areas
- Immunology and Allergy
- Clinical Neurology