TY - JOUR
T1 - Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917
AU - Sivaganesan, Mano
AU - Willis, Jessica R.
AU - Karim, Mohammad
AU - Babatola, Akin
AU - Catoe, David
AU - Boehm, Alexandria B.
AU - Wilder, Maxwell
AU - Green, Hyatt
AU - Lobos, Aldo
AU - Harwood, Valerie J.
AU - Hertel, Stephanie
AU - Klepikow, Regina
AU - Howard, Mondraya F.
AU - Laksanalamai, Pongpan
AU - Roundtree, Alexis
AU - Mattioli, Mia
AU - Eytcheson, Stephanie
AU - Molina, Marirosa
AU - Lane, Molly
AU - Rediske, Richard
AU - Ronan, Amanda
AU - D'Souza, Nishita
AU - Rose, Joan B.
AU - Shrestha, Abhilasha
AU - Hoar, Catherine
AU - Silverman, Andrea I.
AU - Faulkner, Wyatt
AU - Wickman, Kathleen
AU - Kralj, Jason G.
AU - Servetas, Stephanie L.
AU - Hunter, Monique E.
AU - Jackson, Scott A.
AU - Shanks, Orin C.
N1 - Funding Information:
Information has been subjected to U.S. EPA and U.S. CDC peer and administrative review and has been approved for external publication. Any opinions expressed in this paper are those of the authors and do not necessarily reflect the official positions and policies of the U.S. EPA, U.S. Department of Health and Human Services, and U.S. CDC. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use.
Publisher Copyright:
© 2022
PY - 2022/10/15
Y1 - 2022/10/15
N2 - Surface water quality quantitative polymerase chain reaction (qPCR) technologies are expanding from a subject of research to routine environmental and public health laboratory testing. Readily available, reliable reference material is needed to interpret qPCR measurements, particularly across laboratories. Standard Reference Material® 2917 (NIST SRM® 2917) is a DNA plasmid construct that functions with multiple water quality qPCR assays allowing for estimation of total fecal pollution and identification of key fecal sources. This study investigates SRM 2917 interlaboratory performance based on repeated measures of 12 qPCR assays by 14 laboratories (n = 1008 instrument runs). Using a Bayesian approach, single-instrument run data are combined to generate assay-specific global calibration models allowing for characterization of within- and between-lab variability. Comparable data sets generated by two additional laboratories are used to assess new SRM 2917 data acceptance metrics. SRM 2917 allows for reproducible single-instrument run calibration models across laboratories, regardless of qPCR assay. In addition, global models offer multiple data acceptance metric options that future users can employ to minimize variability, improve comparability of data across laboratories, and increase confidence in qPCR measurements.
AB - Surface water quality quantitative polymerase chain reaction (qPCR) technologies are expanding from a subject of research to routine environmental and public health laboratory testing. Readily available, reliable reference material is needed to interpret qPCR measurements, particularly across laboratories. Standard Reference Material® 2917 (NIST SRM® 2917) is a DNA plasmid construct that functions with multiple water quality qPCR assays allowing for estimation of total fecal pollution and identification of key fecal sources. This study investigates SRM 2917 interlaboratory performance based on repeated measures of 12 qPCR assays by 14 laboratories (n = 1008 instrument runs). Using a Bayesian approach, single-instrument run data are combined to generate assay-specific global calibration models allowing for characterization of within- and between-lab variability. Comparable data sets generated by two additional laboratories are used to assess new SRM 2917 data acceptance metrics. SRM 2917 allows for reproducible single-instrument run calibration models across laboratories, regardless of qPCR assay. In addition, global models offer multiple data acceptance metric options that future users can employ to minimize variability, improve comparability of data across laboratories, and increase confidence in qPCR measurements.
KW - Microbial source tracking
KW - Multiple laboratory
KW - Rapid fecal indicator
KW - Standard calibration material
KW - qPCR
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U2 - 10.1016/j.watres.2022.119162
DO - 10.1016/j.watres.2022.119162
M3 - Article
C2 - 36191524
AN - SCOPUS:85139034294
SN - 0043-1354
JO - Water Research
JF - Water Research
ER -