Intra- and inter-visit reproducibility of ganglion cell-inner plexiform layer measurements using handheld optical coherence tomography in children with optic pathway gliomas

Robert A. Avery, Avital Cnaan, Joel S. Schuman, Chieh Li Chen, Natalie C. Glaug, Roger J. Packer, Graham E. Quinn, Hiroshi Ishikawa

    Research output: Contribution to journalArticle

    Abstract

    Purpose: To determine the intra- and inter-visit reproducibility of ganglion cell-inner plexiform layer thickness measures using handheld optical coherence tomography (OCT) in sedated children with optic pathway gliomas and/or neurofibromatosis type 1 (NF1).

    Design: Prospective longitudinal cohort study.

    Methods: Children with sporadic optic pathway gliomas and/or NF1 who had ≥2 volumes acquired over the macula using handheldOCT during sedation for clinically indicated magnetic resonance imaging were eligible for the intra-visit cohort.Children with repeat handheldOCT imagingwithin 6monthswere eligible for the inter-visit cohort. Total retinal thickness and ganglion cell-inner plexiform layer thickness were measured using custom-designed automated segmentation software. Reproducibility was compared across average and anatomic quadrant by calculating the coefficient of variation (CV) and intraclass correlation coefficient (ICC).

    Results: Forty-two subjects (median age 5.4 years, range 0.8-12.7 years) contributed 45 eyes to the intravisit cohort. Thirty-one subject eyes had normal vision and 14 had abnormal vision (decreased visual acuity and/ or visual field). Average and quadrant ganglion cell-inner plexiform layer measures demonstrated CVs ≤4.5% with excellent ICCs (>0.935). The superior quadrant CV differed between subjects with (4.4%) and without (2.1%) vision loss (P <.05). Twenty-five subject eyes were eligible for the inter-visit cohort, demonstrating CVs from 1.6% to 5.2%. Inter-visit ICCs were excellent (0.955-0.995).

    Discussion: Handheld OCT imaging in sedated children with optic pathway gliomas produces highly reproducible measures of ganglion cell-inner plexiform layer thickness.

    Original languageEnglish (US)
    Pages (from-to)916-923.e1
    JournalAmerican Journal of Ophthalmology
    Volume158
    Issue number5
    DOIs
    StatePublished - Nov 1 2014

    ASJC Scopus subject areas

    • Ophthalmology

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