Abstract
Single-molecule function of molecular motors has been well characterized in vitro, but in vivo, motors typically function as part of larger ensembles, including both regulatory proteins and multiple motors. Thus, single-molecule knowledge is only the start of the journey to understand cellular function and organization. The challenge is to use single-molecule information to quantitatively understand transport and its regulation in a cellular context. This article discusses this process focusing on kinesin and dynein, highlighting progress to date, and the challenges involved in using single-molecule function to understand in vivo transport.
Original language | English (US) |
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Title of host publication | Comprehensive Biophysics |
Publisher | Elsevier Inc. |
Pages | 287-297 |
Number of pages | 11 |
Volume | 4 |
ISBN (Print) | 9780080957180 |
DOIs | |
State | Published - 2012 |
Keywords
- BicD
- Dynactin
- Dynein
- Ensemble function
- In vitro
- In vivo
- Kinesin
- Microtubule-based transport
- Molecular motors
- Motor regulation
- Multiple motor
- Regulation
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology