Intracellular transport: Relating single-molecule properties to in vivo function

G. T. Shubeita, S. P. Gross

Research output: Chapter in Book/Report/Conference proceedingChapter


Single-molecule function of molecular motors has been well characterized in vitro, but in vivo, motors typically function as part of larger ensembles, including both regulatory proteins and multiple motors. Thus, single-molecule knowledge is only the start of the journey to understand cellular function and organization. The challenge is to use single-molecule information to quantitatively understand transport and its regulation in a cellular context. This article discusses this process focusing on kinesin and dynein, highlighting progress to date, and the challenges involved in using single-molecule function to understand in vivo transport.

Original languageEnglish (US)
Title of host publicationComprehensive Biophysics
PublisherElsevier Inc.
Number of pages11
ISBN (Print)9780080957180
StatePublished - 2012


  • BicD
  • Dynactin
  • Dynein
  • Ensemble function
  • In vitro
  • In vivo
  • Kinesin
  • Microtubule-based transport
  • Molecular motors
  • Motor regulation
  • Multiple motor
  • Regulation

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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