TY - JOUR
T1 - Intragenic locus in human PIWIL2 gene shares promoter and enhancer functions
AU - Skvortsova, Yulia V.
AU - Kondratieva, Sofia A.
AU - Zinovyeva, Marina V.
AU - Nikolaev, Lev G.
AU - Azhikina, Tatyana L.
AU - Gainetdinov, Ildar V.
N1 - Publisher Copyright:
© 2016 Skvortsova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/6
Y1 - 2016/6
N2 - Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression.
AB - Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression.
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U2 - 10.1371/journal.pone.0156454
DO - 10.1371/journal.pone.0156454
M3 - Article
C2 - 27248499
AN - SCOPUS:84973917501
SN - 1932-6203
VL - 11
JO - PloS one
JF - PloS one
IS - 6
M1 - e0156454
ER -