Intranasal immunization of humans with Streptococcus mutans antigens

F. Li, S. M. Michalek, A. P. Dasanayake, Y. Li, K. Kirk, Noel K. Childers

Research output: Contribution to journalArticle

Abstract

To evaluate the effectiveness of a low dose of soluble or liposomal (L) glucosyltransferase-enriched preparation (E-GTF) in inducing mucosal immune responses after intranasal immunization, 12 adults were immunized on days 0 and 7 by the IN route with 62.5 μg of soluble E-GTF or L-E-GTF. An increase in the mean salivary IgA anti-E-GTF response (P < 0.03) was seen in the L-E-GTF but not the soluble E-GTF group. A significant increase (P < 0.05) in the mean specific IgA antibody activity was also seen in nasal wash from both groups. Although the nasal wash responses were higher in the L-E-GTF than in the soluble E-GTF group, they were not significantly different. The soluble E-GTF immunized group showed a higher serum IgG response than the L-E-GTF immunized group on day 90 (P < 0.05). These results indicate that as little as 62.5 μg of E-GTF, when given by the intranasal route, induced an IgA response in secretions.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalOral Microbiology and Immunology
Volume18
Issue number5
DOIs
StatePublished - Oct 1 2003

Keywords

  • Glucosyltransferase
  • Intranasal immunization
  • Liposome
  • Oral immunity
  • Secretory IgA
  • Streptococcus mutans

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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