Intraocular co-grafts of fetal dorsal raphe nucleus and suprachiasmatic nucleus

Shuichi Ueda, Yasuhiro Matsumoto, Efrain C. Azmitia, Mitsuhiro Kawata

Research output: Contribution to journalArticlepeer-review


Serotonergic neurons in the fetal dorsal raphe nucleus were grafted together with fetal anterior hypothalamic tissue including the suprachiasmatic nucleus (SCN) to the anterior eye chamber of adult rats. After 6 weeks transplantation, the double grafts were immunocytochemically examined using antisera against serotonin, arginine vasopressin (AVP) and vasoactive intestinal polypeptide (VIP). The raphe grafts contained a large number of serotonin-immunoreactive neurons and fibers, but only a few AVP-immunoreactive fibers and VIP-immunoreactive neurons and fibers. On the other hand, numerous AVP- and VIP-immunoreactive neurons and fibers were found in the SCN of the anterior hypothalamic graft. Outgrowing serotonin-immunoreactive fibers from the raphe tissue were densely distributed in the anterior hypothalamic graft. In the SCN, however, only a few fibers were detected. The results demonstrate that the isolated anterior hypothalamic grafts can be innervated by the serotonergic neurons from the raphe grafts, but the innervation pattern of these fibers was quite different from the normal rat. The present results indicate that the isolated SCN has an inhibitory influence on the growth of serotonergic fibers.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalBrain Research
Issue number2
StatePublished - Mar 12 1993


  • Co-graft
  • Dorsal raphe nucleus
  • Immunocytochemistry
  • Serotonin
  • Suprachiasmatic nucleus
  • Vasoactive intestinal polypeptide
  • Vasopressin

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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