Intrinsic bioconjugation for site-specific protein PEGylation at N-terminal serine

Paul M. Levine; Timothy W. Craven; Richard Bonneau; Kent Kirshenbaum

doi:10.1039/c4cc01928h

Intrinsic bioconjugation for site-specific protein PEGylation at N-terminal serine

Paul M. Levine, Timothy W. Craven, Richard Bonneau, Kent Kirshenbaum

Research output: Contribution to journal › Article › peer-review

Abstract

Recently developed chemical ligation protocols were elaborated for rapid N-terminal protein PEGylation. We introduce a PEG-salicylaldehyde ester and demonstrate its site-specific ligation to the N-terminus of the RNase S protein and to the therapeutic polypeptide PTH (1–34).

Original language	English (US)
Pages (from-to)	6909-6912
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	52
DOIs	https://doi.org/10.1039/c4cc01928h
State	Published - Jun 3 2014

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Intrinsic bioconjugation for site-specific protein PEGylation at N-terminal serine",

abstract = "Recently developed chemical ligation protocols were elaborated for rapid N-terminal protein PEGylation. We introduce a PEG-salicylaldehyde ester and demonstrate its site-specific ligation to the N-terminus of the RNase S protein and to the therapeutic polypeptide PTH (1–34).",

author = "Levine, {Paul M.} and Craven, {Timothy W.} and Richard Bonneau and Kent Kirshenbaum",

year = "2014",

month = jun,

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doi = "10.1039/c4cc01928h",

language = "English (US)",

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AU - Levine, Paul M.

AU - Craven, Timothy W.

AU - Bonneau, Richard

AU - Kirshenbaum, Kent

PY - 2014/6/3

Y1 - 2014/6/3

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AB - Recently developed chemical ligation protocols were elaborated for rapid N-terminal protein PEGylation. We introduce a PEG-salicylaldehyde ester and demonstrate its site-specific ligation to the N-terminus of the RNase S protein and to the therapeutic polypeptide PTH (1–34).

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U2 - 10.1039/c4cc01928h

DO - 10.1039/c4cc01928h

M3 - Article

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AN - SCOPUS:84901990070

SN - 1359-7345

VL - 50

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EP - 6912

JO - Chemical Communications

JF - Chemical Communications

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ER -

Intrinsic bioconjugation for site-specific protein PEGylation at N-terminal serine

Abstract

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