Investigation of the application of a Taylor-Couette bioreactor in the post-processing of bioprinted human dermal tissue

Jia Heng Teoh, Anbu Mozhi Thamizhchelvan, Pooya Davoodi, Srinivas Ramasamy, Sanjairaj Vijayavenkataraman, Qiaoyun Yang, Teresa Dicolandrea, Helen Zhao, Jerry Y.H. Fuh, Yih Cherng Liou, Chi Hwa Wang

Research output: Contribution to journalArticle

Abstract

Over the past decade, 3D printing has been explored as a means of producing human skin mimics for applications such as cosmetics testing, drug screening and wound healing. When it comes to the post-processing step of bioprinting skin, choices in recent times have mainly been the static culture of constructs or the use of perfusion and rotatory bioreactors. In this study, we investigated the feasibility of the use of a Taylor-Couette bioreactor in the maturation of bioprinted dermal constructs. Here, a set of bioprinted dermal constructs were cultured in a Taylor-Couette bioreactor up to a period of 7 days and was simultaneously compared with statically cultured constructs. Evaluation of cellular viability using MTS Assay and LIVE/DEAD Assay showed the capability of the Taylor-Couette bioreactor for improving maturation of fibroblasts in the dermal constructs. Histology and immunostaining revealed no adverse effect of the dynamic culture environment on fibroblasts in the constructs. This study thus demonstrates the possibility of utilizing the Taylor-Couette bioreactor as an alternative means for the maturation of the dermal layer in the skin bioprinting pipeline.

Original languageEnglish (US)
Article number107317
JournalBiochemical Engineering Journal
Volume151
DOIs
StatePublished - Nov 15 2019

Keywords

  • Bioprinting
  • Bioreactor
  • Fibroblasts
  • Maturation
  • Taylor-Couette
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Teoh, J. H., Thamizhchelvan, A. M., Davoodi, P., Ramasamy, S., Vijayavenkataraman, S., Yang, Q., Dicolandrea, T., Zhao, H., Fuh, J. Y. H., Liou, Y. C., & Wang, C. H. (2019). Investigation of the application of a Taylor-Couette bioreactor in the post-processing of bioprinted human dermal tissue. Biochemical Engineering Journal, 151, [107317]. https://doi.org/10.1016/j.bej.2019.107317