Isoelectric focusing of brain adenylate cyclase

Douglas J. Franks; Daniel Malamud

doi:10.1016/0003-2697(76)90198-6

Isoelectric focusing of brain adenylate cyclase

Douglas J. Franks, Daniel Malamud

Research output: Contribution to journal › Article › peer-review

Abstract

Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.

Original language	English (US)
Pages (from-to)	486-492
Number of pages	7
Journal	Analytical Biochemistry
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(76)90198-6
State	Published - Jun 1976

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(76)90198-6

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title = "Isoelectric focusing of brain adenylate cyclase",

abstract = "Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.",

author = "Franks, {Douglas J.} and Daniel Malamud",

note = "Funding Information: This study was supported by USPHS Grant GM 21700. The second author holds a National Institutes of Health Career Development Award,",

year = "1976",

month = jun,

doi = "10.1016/0003-2697(76)90198-6",

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AU - Malamud, Daniel

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PY - 1976/6

Y1 - 1976/6

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AB - Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.

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Isoelectric focusing of brain adenylate cyclase

Abstract

ASJC Scopus subject areas

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