Isoelectric focusing of brain adenylate cyclase

Douglas J. Franks, Daniel Malamud

    Research output: Contribution to journalArticlepeer-review


    Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.

    Original languageEnglish (US)
    Pages (from-to)486-492
    Number of pages7
    JournalAnalytical Biochemistry
    Issue number2
    StatePublished - Jun 1976

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology


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