Abstract
Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.
Original language | English (US) |
---|---|
Pages (from-to) | 486-492 |
Number of pages | 7 |
Journal | Analytical Biochemistry |
Volume | 73 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1976 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology