Isoelectric focusing of brain adenylate cyclase

Douglas J. Franks, Daniel Malamud

Research output: Contribution to journalArticlepeer-review

Abstract

Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.

Original languageEnglish (US)
Pages (from-to)486-492
Number of pages7
JournalAnalytical Biochemistry
Volume73
Issue number2
DOIs
StatePublished - Jun 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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