@article{11d1770fdfcb433a94378502f7db320f,
title = "Isoform discovery by targeted cloning, 'deep-well' pooling and parallel sequencing",
abstract = "Describing the 'ORFeome' of an organism, including all major isoforms, is essential for a system-level understanding of any species; however, conventional cloning and sequencing approaches are prohibitively costly and labor-intensive. We describe a potentially genome-wide methodology for efficiently capturing new coding isoforms using reverse transcriptase (RT)-PCR recombinational cloning, 'deep-well' pooling and a next-generation sequencing platform. This ORFeome discovery pipeline will be applicable to any eukaryotic species with a sequenced genome.",
author = "Kourosh Salehi-Ashtiani and Xinping Yang and Adnan Derti and Weidong Tian and Tong Hao and Chenwei Lin and Kathryn Makowski and Lei Shen and Murray, {Ryan R.} and David Szeto and Nadeem Tusneem and Smith, {Douglas R.} and Cusick, {Michael E.} and Hill, {David E.} and Roth, {Frederick P.} and Marc Vidal",
note = "Funding Information: This work was funded in part by a grant from the Ellison Foundation (awarded to M.V.) and in part by the Dana Farber Cancer Institute Strategic Initiative in support of Center for Cancer Systems Biology. F.P.R. acknowledges support from US National Institutes of Health grants NS054052, HG003224, HL081341. W.T. was supported in part by National Institutes of Health grant DK070078. We thank the West Quad Computing Group at Harvard Medical School as well as Research Computing at Massachusetts General Hospital for assistance with computational resources. We thank G. Temple for helpful comments on the manuscript.",
year = "2008",
month = jul,
doi = "10.1038/nmeth.1224",
language = "English (US)",
volume = "5",
pages = "597--600",
journal = "Nature methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "7",
}