Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins

Raffaello Verardi, Nathaniel J. Traaseth, Larry R. Masterson, Vitaly V. Vostrikov, Gianluigi Veglia

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter, we summarize the isotopic labeling strategies used to obtain high-quality solution and solid-state NMR spectra of biological samples, with emphasis on integral membrane proteins (IMPs). While solution NMR is used to study IMPs under fast tumbling conditions, such as in the presence of detergent micelles or isotropic bicelles, solid-state NMR is used to study the structure and orientation of IMPs in lipid vesicles and bilayers. In spite of the tremendous progress in biomolecular NMR spectroscopy, the homogeneity and overall quality of the sample is still a substantial obstacle to overcome. Isotopic labeling is a major avenue to simplify overlapped spectra by either diluting the NMR active nuclei or allowing the resonances to be separated in multiple dimensions. In the following we will discuss isotopic labeling approaches that have been successfully used in the study of IMPs by solution and solid-state NMR spectroscopy.

Original languageEnglish (US)
Title of host publicationIsotope labeling in Biomolecular NMR
EditorsHanudatta S. Atreya
Pages35-62
Number of pages28
DOIs
StatePublished - 2012

Publication series

NameAdvances in Experimental Medicine and Biology
Volume992
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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