L1 drives IFN in senescent cells and promotes age-associated inflammation

Marco De Cecco, Takahiro Ito, Anna P. Petrashen, Amy E. Elias, Nicholas J. Skvir, Steven W. Criscione, Alberto Caligiana, Greta Brocculi, Emily M. Adney, Jef D. Boeke, Oanh Le, Christian Beauséjour, Jayakrishna Ambati, Kameshwari Ambati, Matthew Simon, Andrei Seluanov, Vera Gorbunova, P. Eline Slagboom, Stephen L. Helfand, Nicola NerettiJohn M. Sedivy

Research output: Contribution to journalArticlepeer-review


Retrotransposable elements are deleterious at many levels, and the failure of host surveillance systems for these elements can thus have negative consequences. However, the contribution of retrotransposon activity to ageing and age-associated diseases is not known. Here we show that during cellular senescence, L1 (also known as LINE-1) retrotransposable elements become transcriptionally derepressed and activate a type-I interferon (IFN-I) response. The IFN-I response is a phenotype of late senescence and contributes to the maintenance of the senescence-associated secretory phenotype. The IFN-I response is triggered by cytoplasmic L1 cDNA, and is antagonized by inhibitors of the L1 reverse transcriptase. Treatment of aged mice with the nucleoside reverse transcriptase inhibitor lamivudine downregulated IFN-I activation and age-associated inflammation (inflammaging) in several tissues. We propose that the activation of retrotransposons is an important component of sterile inflammation that is a hallmark of ageing, and that L1 reverse transcriptase is a relevant target for the treatment of age-associated disorders.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
Issue number7742
StatePublished - Feb 7 2019

ASJC Scopus subject areas

  • General


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