TY - JOUR
T1 - Label-free microfluidic stem cell isolation technologies
AU - Menachery, Anoop
AU - Kumawat, Nityanand
AU - Qasaimeh, Mohammad
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Stem cell research has great potential in clinical applications and regenerative medicine, which highlight the need for efficient stem cell isolation technologies. Enrichment of stem cells is challenging because of its sensitivity to the surrounding environment and its overlapping features with non-target cells. Current gold-standard isolation techniques depend on surface antigens that are exclusively expressed on stem cells, where surface immuno-labeling is followed by flow cytometry or magnetic sorting procedures. It is well-established that stem cells should be enriched with minimal alteration, therefore, isolation without immunological tagging in a label-free format is of great interest. Microfluidic technologies have demonstrated high level of control to precisely manipulate stem cells. Here, we review the recent emerging label-free microfluidic based cell isolation technologies that provide unbiased stem cell enrichment based on their size and deformability, adhesion, and electrical characteristics, and discuss their potential to achieve effective stem cell isolation for research and clinical applications.
AB - Stem cell research has great potential in clinical applications and regenerative medicine, which highlight the need for efficient stem cell isolation technologies. Enrichment of stem cells is challenging because of its sensitivity to the surrounding environment and its overlapping features with non-target cells. Current gold-standard isolation techniques depend on surface antigens that are exclusively expressed on stem cells, where surface immuno-labeling is followed by flow cytometry or magnetic sorting procedures. It is well-established that stem cells should be enriched with minimal alteration, therefore, isolation without immunological tagging in a label-free format is of great interest. Microfluidic technologies have demonstrated high level of control to precisely manipulate stem cells. Here, we review the recent emerging label-free microfluidic based cell isolation technologies that provide unbiased stem cell enrichment based on their size and deformability, adhesion, and electrical characteristics, and discuss their potential to achieve effective stem cell isolation for research and clinical applications.
KW - Cell isolation
KW - Label-free
KW - Microfluidics
KW - Single cell
KW - Stem cell
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U2 - 10.1016/j.trac.2017.01.008
DO - 10.1016/j.trac.2017.01.008
M3 - Review article
AN - SCOPUS:85012104114
VL - 89
SP - 1
EP - 12
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
SN - 0165-9936
ER -