Laddec® versus Bio-Oss®: The effect on the healing of critical-sized defect – Calvaria rabbit model

Luiz Fernando Gil, Vasudev Vivekanand Nayak, Ernesto B. Benalcázar Jalkh, Nick Tovar, Kai Jen Chiu, Jaime Campos Salas, Charles Marin, Michelle Bowers, Gileade Freitas, Dejolie Christelle Mbe Fokam, Paulo G. Coelho, Lukasz Witek

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to evaluate the in vivo performance of two different deproteinized bovine bone (DBB) grafting materials: DBBB (Bio-Oss®) and DBBL (Laddec®), for the regeneration of critically sized (8 mm) defects in rabbit's calvaria. Three round-shaped defects were surgically created in the calvaria of 13 New Zealand White rabbits proximal to the coronal suture in the parietal bone. Two of the defects were filled with one of the grafting materials while a third was left empty to serve as a negative control. Bone regeneration properties were evaluated at 4- and 8-weeks after implantation by means of histological and histomorphometrical analyses. Statistical analyses were performed through a mixed model analysis with fixed factors of time and material. Histological evaluation of the control group evidenced a lack of bridging bone formation across the defect sites at both evaluation time points. For the experimental groups, new bone formation was observed around the defect periphery and to progress radially inwards to the center of the defect site, regardless of the grafting material. Histomorphometric analyses at 4 weeks demonstrated higher amount of bone formation through the defect for DBBB group. However, at 8 weeks, DBBL and DBBB demonstrated osteoconductivity and low resorption rates with evidence of statistically similar bone regeneration through the complete boney defect. Finally, DBBB presented lower soft tissue migration within the defect when compared to DBBL at both evaluation time points. DBBB and DBBL presented similar bone regeneration performance and slow resorption rates. Although both materials promoted bone regeneration through the complete defect, DBBB presented lower soft tissue migration within the defects at 4- and 8-weeks.

Original languageEnglish (US)
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
StateAccepted/In press - 2022

Keywords

  • bone graft
  • bone regeneration
  • deproteinized bovine bone graft
  • osteoconduction
  • xenograft

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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