Laser spectroscopic studies of interactions of UVI with bacterial phosphate species

Roger Knopp, Petra J. Panak, Lewis A. Wray, Neil S. Renninger, Jay D. Keasling, Heino Nitsche

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have investigated the interactions of UVI with two bacterial phosphate-containing species: Gram-positive Bacillus sphaericus and Gram-negative Psedomonas aeruginosa. The Gram-positive B. sphaericus was investigated by using Raman spectroscopy and time-resolved laser-induced fluorescence spectroscopy (TRLFS). We found that living cells, spores, and intact heat-killed cells complexed UVI (pH4.5) through phosphate groups bound to their surfaces, while decomposed cells released H2PO4- and precipitated UVI as UO2(H2PO4)2. TRLFS of UVI showed that Gram-negative P. aeruginosa - genetically engineered to accumulate polyphosphate, subsequently degrade it, and secrete phosphate - precipitated UVI quantitatively at pH 4.5, The same bacterial strain, not induced to secrete phosphate, sorbed only a small amount of UVI.

    Original languageEnglish (US)
    Pages (from-to)2812-2818
    Number of pages7
    JournalChemistry - A European Journal
    Volume9
    Issue number12
    DOIs
    StatePublished - Jun 16 2003

    Keywords

    • Bacteria
    • Fluorescence spectroscopy
    • Phosphate
    • Raman spectroscopy
    • Sorption
    • Uranium

    ASJC Scopus subject areas

    • Catalysis
    • Organic Chemistry

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