@article{a7051457b18d48839d370495823ddbbe,
title = "Layer I Interneurons Sharpen Sensory Maps during Neonatal Development",
abstract = "The neonatal mammal faces an array of sensory stimuli when diverse neuronal types have yet to form sensory maps. How these inputs interact with intrinsic neuronal activity to facilitate circuit assembly is not well understood. By using longitudinal calcium imaging in unanesthetized mouse pups, we show that layer I (LI) interneurons, delineated by co-expression of the 5HT3a serotonin receptor (5HT3aR) and reelin (Re), display spontaneous calcium transients with the highest degree of synchrony among cell types present in the superficial barrel cortex at postnatal day 6 (P6). 5HT3aR Re interneurons are activated by whisker stimulation during this period, and sensory deprivation induces decorrelation of their activity. Moreover, attenuation of thalamic inputs through knockdown of NMDA receptors (NMDARs) in these interneurons results in expansion of whisker responses, aberrant barrel map formation, and deficits in whisker-dependent behavior. These results indicate that recruitment of specific interneuron types during development is critical for adult somatosensory function.",
keywords = "barrel cortex, calcium imaging, development, interneuron, layer I, spontaneous activity, thalamocortical connectivity",
author = "Alicia Che and Rachel Babij and Iannone, {Andrew F.} and Fetcho, {Robert N.} and Monica Ferrer and Conor Liston and Gord Fishell and {De Marco Garc{\'i}a}, {Natalia V.}",
note = "Funding Information: We are grateful to Z. Duan, P. Chu, J. Kaltschmidt, J. Ackman, D. Pisapia, and T. Ryan for comments on the manuscript. We thank J. Witztum for help with the whisker stimulation and imaging setup, W. Gan and B. Rudy for helpful discussions, and T. Otsuka and S. Baksh for technical assistance. In addition, we recognize the generosity of the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Research Campus for making GCaMP6s available. This work was supported by grants from NIH (NIMH 1R01MH110553-01), Pediatric Epilepsies Award (Citizens United for Research in Epilepsy), Leon Levy Fellowship Award (Leon Levy Foundation), and Frueauff Foundation Award to N.V.D.M.G.; NIMH (1R01 MH109685) to C.L.; and NIGMS (T32GM007739) to Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program in support for R.B., A.F.I., and R.N.F. Funding Information: We are grateful to Z. Duan, P. Chu, J. Kaltschmidt, J. Ackman, D. Pisapia, and T. Ryan for comments on the manuscript. We thank J. Witztum for help with the whisker stimulation and imaging setup, W. Gan and B. Rudy for helpful discussions, and T. Otsuka and S. Baksh for technical assistance. In addition, we recognize the generosity of the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Research Campus for making GCaMP6s available. This work was supported by grants from NIH ( NIMH 1R01MH110553-01 ), Pediatric Epilepsies Award ( Citizens United for Research in Epilepsy ), Leon Levy Fellowship Award ( Leon Levy Foundation ), and Frueauff Foundation Award to N.V.D.M.G.; NIMH ( 1R01 MH109685 ) to C.L.; and NIGMS ( T32GM007739 ) to Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program in support for R.B., A.F.I., and R.N.F. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
month = jul,
day = "11",
doi = "10.1016/j.neuron.2018.06.002",
language = "English (US)",
volume = "99",
pages = "98--116.e7",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}