Lego-like Microfluidic Probe for concentration gradient discretization: a path toward complete modularization

Here, we introduce systems and methods aimed at achieving complete modularization of the microfluidic probe (MFP) technology.This is accomplished by breaking down the MFP structure into simple fundamental units that can be assembled in various combinations to serve multiple applications concurrently.We employ a Lego-like tight-fit mechanism to ensure precise maintenance of flow channel continuity.Termed the Modular MFP, as a proof of concept, we propose one design capable of mixing two reagents and discretizing their concentration gradient.With the ability to swap units, the Modular MFP can generate 3, 6, and 12 distinct mix concentrations and seamlessly switch between them in-situ.The ultimate objective of this modularization effort is to standardize the technology and enhance its user-friendliness, enabling biologists and researchers in the medical field to utilize it effectively.We anticipate that modularization will propel MFP technology towards realizing its long-awaited potential as a versatile tool with superior capabilities.