TY - JOUR
T1 - Light-activated serotonin for exploring its action in biological systems
AU - Rea, Adam C.
AU - Vandenberg, Laura N.
AU - Ball, Rebecca E.
AU - Snouffer, Ashley A.
AU - Hudson, Alicia G.
AU - Zhu, Yue
AU - McLain, Duncan E.
AU - Johnston, Lindsey L.
AU - Lauderdale, James D.
AU - Levin, Michael
AU - Dore, Timothy M.
N1 - Funding Information:
We thank Matthew J. O’Connor for technical assistance. This work was supported by grants from the National Institutes of Health (R01 NS070159 to J.D.L. and T.M.D. and GM077425 to M.L.), the American Heart Association (0740088N to M.L.), and the National Science Foundation (CHE-1012412 and CHE-1317760 to T.M.D.).
PY - 2013/12/19
Y1 - 2013/12/19
N2 - Summary Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.
AB - Summary Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.
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U2 - 10.1016/j.chembiol.2013.11.005
DO - 10.1016/j.chembiol.2013.11.005
M3 - Article
C2 - 24333002
AN - SCOPUS:84890859856
SN - 1074-5521
VL - 20
SP - 1536
EP - 1546
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 12
ER -