Light-mediated inhibition of protein synthesis

Michael Goard, Girish Aakalu, Olesya D. Fedoryak, Carlo Quinonez, Jamii St. Julien, Stephen J. Poteet, Erin M. Schuman, Timothy M. Dore

Research output: Contribution to journalArticle

Abstract

The regulation of protein synthesis is vital for a host of cell biological processes, but investigating roles for protein synthesis have been hindered by the inability to selectively interfere with it. To inhibit protein synthesis with spatial and temporal control, we have developed a photo-releasable anisomycin compound, N-([6-bromo-7-hydroxycoumarin-4-yl]methyloxycarbonyl) anisomycin (Bhc-Aniso), that can be removed through exposure to UV light. The area of protein synthesis inhibition can be restricted to a small light-exposed region or, potentially, the volume of two-photon excitation if a pulsed IR laser is the light source. We have tested the compound's effectiveness with an in vitro protein-translation system, CHO cells, HEK293 cells, and neurons. The photo-released anisomycin can inhibit protein synthesis in a spatially restricted manner, which will enable the specific inhibition of protein synthesis in subsets of cells with temporal and spatial precision.

Original languageEnglish (US)
Pages (from-to)685-693
Number of pages9
JournalChemistry and Biology
Volume12
Issue number6
DOIs
StatePublished - 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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  • Cite this

    Goard, M., Aakalu, G., Fedoryak, O. D., Quinonez, C., St. Julien, J., Poteet, S. J., Schuman, E. M., & Dore, T. M. (2005). Light-mediated inhibition of protein synthesis. Chemistry and Biology, 12(6), 685-693. https://doi.org/10.1016/j.chembiol.2005.04.018