@article{0b010dd8d5234905aaa68c8a24e39d63,
title = "LINE-1 protein localization and functional dynamics during the cell cycle",
abstract = "LINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology. However, a clear understanding of L1{\textquoteleft}s lifecycle and the processes involved in restricting its insertion and intragenomic spread remains elusive. Here we identify modes of L1 proteins{\textquoteright} entrance into the nucleus, a necessary step for L1 proliferation. Using functional, biochemical, and imaging approaches, we also show a clear cell cycle bias for L1 retrotransposition that peaks during the S phase. Our observations provide a basis for novel interpretations about the nature of nuclear and cytoplasmic L1 ribonucleoproteins (RNPs) and the potential role of DNA replication in L1 retrotransposition.",
author = "Paolo Mita and Aleksandra Wudzinska and Xiaoji Sun and Joshua Andrade and Shruti Nayak and Kahler, {David J.} and Sana Badri and John LaCava and Beatrix Ueberheide and Yun, {Chi Y.} and David Feny{\"o} and Boeke, {Jef D.}",
note = "Funding Information: This work was supported by NIH grant P50GM107632 to JDB. The cytometry and cell sorting, High Throughput Biology (HTB) and Proteomic cores are partially supported by Laura and Isaac Perlmut- ter Cancer Center Support Grant, (NIH/NCI P30CA16087) and NYSTEM Contract C026719 (HTB core) and NIH/ORIP 1S10OD010582 grant (proteomic core). We thank Gregory Brittingham and Dr. Liam Holt for their help in collecting confocal images and movies and Dr. Timothee Lionnet for the help with RNA FISH. The model presented in Figure 10 was constructed modifying available pictures from mindthegraph-Science Infographic Maker. National Institutes of Health P50GM107632 Jef D Boeke National Cancer Institute NIH/NCI P30CA16087 Chi Y Yun National Institutes of Health 1S10OD010582 Chi Y Yun NYSTEM Contract C026719 Chi Y Yun Funding Information: This work was supported by NIH grant P50GM107632 to JDB. The cytometry and cell sorting, High Throughput Biology (HTB) and Proteomic cores are partially supported by Laura and Isaac Perlmut-ter Cancer Center Support Grant, (NIH/NCI P30CA16087) and NYSTEM Contract C026719 (HTB core) and NIH/ORIP 1S10OD010582 grant (proteomic core). We thank Gregory Brittingham and Dr. Liam Holt for their help in collecting confocal images and movies and Dr. Timothee Lionnet for the help with RNA FISH. The model presented in Figure 10 was constructed modifying available pictures from mindthegraph-Science Infographic Maker. Publisher Copyright: {\textcopyright} Mita et al.",
year = "2018",
month = jan,
day = "8",
doi = "10.7554/eLife.30058",
language = "English (US)",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}