Liquid–liquid phase separation of the Golgi matrix protein GM130

Aleksander A. Rebane, Pascal Ziltener, Lauren C. LaMonica, Antonia H. Bauer, Hong Zheng, Iván López-Montero, Frederic Pincet, James E. Rothman, Andreas M. Ernst

Research output: Contribution to journalArticlepeer-review


Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50–400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as vesicle tethers, Golgins may also possess the capacity to phase separate and, thereby, contribute to the internal organization of the Golgi. GM130 is the most abundant Golgin at the cis Golgi. Remarkably, overexpressed GM130 forms liquid droplets in cells analogous to those described for numerous intrinsically disordered proteins with low complexity sequences, even though GM130 is neither low in complexity nor intrinsically disordered. Virtually pure recombinant GM130 also phase-separates into dynamic, liquid-like droplets in close to physiological buffers and at concentrations similar to its estimated local concentration at the cis Golgi.

Original languageEnglish (US)
Pages (from-to)1132-1144
Number of pages13
JournalFEBS Letters
Issue number7
StatePublished - Apr 1 2020


  • Golgi matrix
  • Golgin
  • coiled-coil
  • liquid
  • liquid phase separation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'Liquid–liquid phase separation of the Golgi matrix protein GM130'. Together they form a unique fingerprint.

Cite this