@article{7530d7cd26da4d62b657589b3e9fd5a0,
title = "Liquid–liquid phase separation of the Golgi matrix protein GM130",
abstract = "Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50–400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as vesicle tethers, Golgins may also possess the capacity to phase separate and, thereby, contribute to the internal organization of the Golgi. GM130 is the most abundant Golgin at the cis Golgi. Remarkably, overexpressed GM130 forms liquid droplets in cells analogous to those described for numerous intrinsically disordered proteins with low complexity sequences, even though GM130 is neither low in complexity nor intrinsically disordered. Virtually pure recombinant GM130 also phase-separates into dynamic, liquid-like droplets in close to physiological buffers and at concentrations similar to its estimated local concentration at the cis Golgi.",
keywords = "Golgi matrix, Golgin, coiled-coil, liquid, liquid phase separation",
author = "Rebane, {Aleksander A.} and Pascal Ziltener and LaMonica, {Lauren C.} and Bauer, {Antonia H.} and Hong Zheng and Iv{\'a}n L{\'o}pez-Montero and Frederic Pincet and Rothman, {James E.} and Ernst, {Andreas M.}",
note = "Funding Information: This work was supported by an NIH grant to JER (GM R35 118084). We thank Al Mennone at the Yale Center for Cellular and Molecular Imaging Facility (CCMI, NIH grant S10OD020142), and Yuling Lei for technical assistance with protein purification. IL-M acknowledges the Fulbright Foundation and MECD for a sabbatical stay (CAS17/00304) at Yale. We thank Josh Lees and Xiaolei Su for valuable discussions, and Ethan Perets for gifting us the purified Ddx4 fragment. Funding Information: This work was supported by an NIH grant to JER (GM R35 118084). We thank Al Mennone at the Yale Center for Cellular and Molecular Imaging Facility (CCMI, NIH grant S10OD020142), and Yuling Lei for technical assistance with protein purification. IL‐M acknowledges the Fulbright Foundation and MECD for a sabbatical stay (CAS17/00304) at Yale. We thank Josh Lees and Xiaolei Su for valuable discussions, and Ethan Perets for gifting us the purified Ddx4 fragment. Publisher Copyright: {\textcopyright} 2019 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies",
year = "2020",
month = apr,
day = "1",
doi = "10.1002/1873-3468.13715",
language = "English (US)",
volume = "594",
pages = "1132--1144",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "7",
}