Local definition of Ty1 target preference by long terminal repeats and clustered tRNA genes

Nurjana Bachmann, Yolanda Eby, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    LTR-containing retrotransposons reverse transcribe their RNA genomes, and the resulting cDNAs are integrated into the genome by the element-encoded integrase protein. The yeast LTR retrotransposon Ty1 preferentially integrates into a target window upstream of tDNAs (tRNA genes) in the yeast genome. We investigated the nature of these insertions and the target window on a genomic scale by analyzing several hundred de novo insertions upstream of tDNAs in two different multicopy gene families. The pattern of insertion upstream of tDNAs was nonrandom and periodic, with peaks separated by -80 bp. Insertions were not distributed equally throughout the genome, as certain tDNAs within a given family received higher frequencies of upstream Ty1 insertions than others. We showed that the presence and relative position of additional tDNAs and LTRs surrounding the target tDNA dramatically influenced the frequency of insertion events upstream of that target.

    Original languageEnglish (US)
    Pages (from-to)1232-1247
    Number of pages16
    JournalGenome Research
    Volume14
    Issue number7
    DOIs
    StatePublished - Jul 2004

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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