@inproceedings{312a3c20a5e34431aa9152e7585673cd,
title = "Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping",
abstract = "We developed an optofluidic biosensor by integrating a microfluidic device into a Localized Surface Plasmon Resonance (LSPR) detection system to quantitatively analyze a cell-secreted cytokine, TNF-alpha. The device was designed for on-chip trapping and incubation of immune cells and subsequent detection of cytokines by a gold nanoparticle-based LSPR sensor. During the 2 hour incubation of 50,000 THP-1 cells in the microfluidic device, we observed a shift of 0.2877nm in the LSPR surface absorbance spectrum peak, which corresponds to a TNF-alpha concentration of 327ng/mL. This device enables LSPR technique for cell-secreted cytokine assay detection by scaling down the cell secretion volume.",
keywords = "Biosensor, Localized surface plasmon resonance (LSPR), Optofluidic",
author = "Oh, {Bo Ram} and Huang, {Nien Tsu} and Weiqiang Chen and Jungwhan Seo and Jianping Fu and Katsuo Kurabayashi",
year = "2013",
language = "English (US)",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "92--94",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}