Long term depletion of serotonin leads to selective changes in glutamate receptor subunits

Fumihiro Shutoh, Shun Hamada, Michiyo Shibata, Masaaki Narita, Takashi Shiga, Efrain C. Azmitia, Nobuo Okado

Research output: Contribution to journalArticle

Abstract

The present study was carried out to clarify possible modulation mechanism of serotonin (5-HT) on glutamatergic neurotransmission in the rat cerebral cortex. 5-HT was depleted by a 5-HT metabolite blocker (para-chlorophenylalanine; pCPA) for a week. Receptor binding experiments using (S)-[3H]α-amino-3-hydroxy-5-methylisoxazol-4-propionic acid (AMPA) showed a considerable increase in Bmax value of the membrane samples prepared from the cerebral cortex of rats compared with that of control animals received saline. In contrast, Bmax value of the [3H]MK-801 binding experiments for NMDA receptor was not changed by pCPA-treatment. Changes in the density of each AMPA receptor subtype were examined in the cerebral cortex by immunoblot analyses using antibodies against AMPA receptor subunits. The density of immunoreactive bands with receptor subtype specific antibodies against GluR2/3 and GluR2 receptors was increased, whereas that of GluR1 receptors was decreased. Considering GluR2 receptor subtype inhibits Ca2+ influx into neurons, the present study suggests that 5-HT appears to modulate synaptic plasticity by regulating the density of each AMPA receptor subtype.

Original languageEnglish (US)
Pages (from-to)365-371
Number of pages7
JournalNeuroscience Research
Volume38
Issue number4
DOIs
StatePublished - 2000

Keywords

  • (S)-[3H]α-amino-3-hydroxy-5-methylisoxazol-4-propionic acid (AMPA)
  • Excitatory amino acid
  • Glutamate receptor
  • Plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

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