Loss of dna methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling

Yelena Chernyavskaya, Raksha Mudbhary, Chi Zhang, Debra Tokarz, Vinitha Jacob, Smita Gopinath, Xiaochen Sun, Shuang Wang, Elena Magnani, Bhavani P. Madakashira, Jeffrey A. Yoder, Yujin Hoshida, Kirsten C. Sadler

Research output: Contribution to journalArticle

Abstract

Complex cytoplasmic nucleotide-sensing mechanisms can recognize foreign DNA based on a lack of methylation and initiate an immune response to clear the infection. Zebrafish embryos with global DNA hypomethylation caused by mutations in the ubiquitin-like with PHD and ring finger domains 1 (uhrf1) or DNA methyltransferase 1 (dnmt1) genes exhibit a robust interferon induction characteristic of the first line of defense against viral infection. We found that this interferon induction occurred in non-immune cells and examined whether intracellular viral sensing pathways in these cells were the trigger. RNA-seq analysis of uhrf1 and dnmt1 mutants revealed widespread induction of Class I retrotransposons and activation of cytoplasmic DNA viral sensors. Attenuating Sting, phosphorylated Tbk1 and, importantly, blocking reverse transcriptase activity suppressed the expression of interferon genes in uhrf1 mutants. Thus, activation of transposons in cells with global DNA hypomethylation mimics a viral infection by activating cytoplasmic DNA sensors. This suggests that antiviral pathways serve as surveillance of cells that have derepressed intragenomic parasites due to DNA hypomethylation.

Original languageEnglish (US)
Pages (from-to)2925-2939
Number of pages15
JournalDevelopment (Cambridge)
Volume144
Issue number16
DOIs
StatePublished - Aug 15 2017

Keywords

  • Antiviral
  • DNA methylation
  • Interferon
  • Transposon
  • Uhrf1

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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  • Cite this

    Chernyavskaya, Y., Mudbhary, R., Zhang, C., Tokarz, D., Jacob, V., Gopinath, S., Sun, X., Wang, S., Magnani, E., Madakashira, B. P., Yoder, J. A., Hoshida, Y., & Sadler, K. C. (2017). Loss of dna methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling. Development (Cambridge), 144(16), 2925-2939. https://doi.org/10.1242/dev.147629