Lymphocyte subpopulations in the neonate: A subset of HNK-1-, OKT3-, OKT8+ lymphocytes displays natural killer activity

Antonella Vitiello; Rita Maccario; Daniela Montagna; Fulvio A. Porta; Cristina M. Alberini; Gabriella Minorat; Giulia C.B. Astaldi-Ricotti; Luigi Nespoli; Alberto G. Ugazio

doi:10.1016/0008-8749(84)90295-8

Lymphocyte subpopulations in the neonate: A subset of HNK-1^-, OKT3^-, OKT8⁺ lymphocytes displays natural killer activity

Antonella Vitiello, Rita Maccario, Daniela Montagna, Fulvio A. Porta, Cristina M. Alberini, Gabriella Minorat, Giulia C.B. Astaldi-Ricotti, Luigi Nespoli, Alberto G. Ugazio

Neural Science

Research output: Contribution to journal › Article › peer-review

Abstract

It has been recently reported that cord blood lymphocytes (CBL) contain a subpopulation of OKT8⁺, sheep erythrocyte-rosetting negative (E^-) cells not detectable in adult peripheral blood lymphocytes (a-PBL). The present studies were undertaken to characterize this subset of lymphocytes functionally and phenotypically. OKT8⁺ cells were purified from E-depleted CBL by negative selection on nylon-wool columns as well as by positive selection on plates coated with rabbit antibody to murine IgG (panning). The purified CBL displayed natural killer (NK) activity against K562 erythroleukemic cells. Although most of these CBL were large granular lymphocytes, they lacked typical NK markers such as HNK-1 and OKM1 surface antigens. Most OKT8⁺, OKT3^- cells were also OKT10⁺, Ia⁺ and had the receptor for peanut agglutinin. These CBL may represent a stage along the differentiation pathway leading to mature NK or T cells.

Original language	English (US)
Pages (from-to)	252-257
Number of pages	6
Journal	Cellular Immunology
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(84)90295-8
State	Published - Apr 15 1984

ASJC Scopus subject areas

Immunology

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@article{6a7798ec607348849722a70316bd2111,

title = "Lymphocyte subpopulations in the neonate: A subset of HNK-1-, OKT3-, OKT8+ lymphocytes displays natural killer activity",

abstract = "It has been recently reported that cord blood lymphocytes (CBL) contain a subpopulation of OKT8+, sheep erythrocyte-rosetting negative (E-) cells not detectable in adult peripheral blood lymphocytes (a-PBL). The present studies were undertaken to characterize this subset of lymphocytes functionally and phenotypically. OKT8+ cells were purified from E-depleted CBL by negative selection on nylon-wool columns as well as by positive selection on plates coated with rabbit antibody to murine IgG (panning). The purified CBL displayed natural killer (NK) activity against K562 erythroleukemic cells. Although most of these CBL were large granular lymphocytes, they lacked typical NK markers such as HNK-1 and OKM1 surface antigens. Most OKT8+, OKT3- cells were also OKT10+, Ia+ and had the receptor for peanut agglutinin. These CBL may represent a stage along the differentiation pathway leading to mature NK or T cells.",

author = "Antonella Vitiello and Rita Maccario and Daniela Montagna and Porta, {Fulvio A.} and Alberini, {Cristina M.} and Gabriella Minorat and Astaldi-Ricotti, {Giulia C.B.} and Luigi Nespoli and Ugazio, {Alberto G.}",

note = "Funding Information: {\textquoteright} This work was supported, in part, by CNR, Rome. * Current address: Department of Immunology, Research Institute of Scripps Clinic, Scripps Clinic and Research Foundation, La Jolla, Calif. 92037. 3 Current address: Institute of Genetics of CNR, 27 100 Pavia, Italy. 4 To whom correspondence should be addressed: Dipartimento di Pediatria, Universiti di Pavia, Ospedale S. Matteo, P.le Golgi, 27100 Pavia, Italy. 5 Abbreviations used in this paper: AET, 2-aminoethylisotbiouronium bromide; BSA, bovine serum albumin; CBL, cord blood lymphocytes; E, E-rosette-forming cells; FCS, fetal calf serum; B-2, Interleukin 2; LGL, large granular lymphocytes; NK, natural killer; a-PBL, adult peripheral blood lymphocytes; PBS, phosphate-buffered saline; PNA, peanut agglutinin; TdT, deoxynucleotidyl transferase.",

year = "1984",

month = apr,

day = "15",

doi = "10.1016/0008-8749(84)90295-8",

language = "English (US)",

volume = "85",

pages = "252--257",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

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TY - JOUR

T1 - Lymphocyte subpopulations in the neonate

T2 - A subset of HNK-1-, OKT3-, OKT8+ lymphocytes displays natural killer activity

AU - Vitiello, Antonella

AU - Maccario, Rita

AU - Montagna, Daniela

AU - Porta, Fulvio A.

AU - Alberini, Cristina M.

AU - Minorat, Gabriella

AU - Astaldi-Ricotti, Giulia C.B.

AU - Nespoli, Luigi

AU - Ugazio, Alberto G.

N1 - Funding Information: ’ This work was supported, in part, by CNR, Rome. * Current address: Department of Immunology, Research Institute of Scripps Clinic, Scripps Clinic and Research Foundation, La Jolla, Calif. 92037. 3 Current address: Institute of Genetics of CNR, 27 100 Pavia, Italy. 4 To whom correspondence should be addressed: Dipartimento di Pediatria, Universiti di Pavia, Ospedale S. Matteo, P.le Golgi, 27100 Pavia, Italy. 5 Abbreviations used in this paper: AET, 2-aminoethylisotbiouronium bromide; BSA, bovine serum albumin; CBL, cord blood lymphocytes; E, E-rosette-forming cells; FCS, fetal calf serum; B-2, Interleukin 2; LGL, large granular lymphocytes; NK, natural killer; a-PBL, adult peripheral blood lymphocytes; PBS, phosphate-buffered saline; PNA, peanut agglutinin; TdT, deoxynucleotidyl transferase.

PY - 1984/4/15

Y1 - 1984/4/15

N2 - It has been recently reported that cord blood lymphocytes (CBL) contain a subpopulation of OKT8+, sheep erythrocyte-rosetting negative (E-) cells not detectable in adult peripheral blood lymphocytes (a-PBL). The present studies were undertaken to characterize this subset of lymphocytes functionally and phenotypically. OKT8+ cells were purified from E-depleted CBL by negative selection on nylon-wool columns as well as by positive selection on plates coated with rabbit antibody to murine IgG (panning). The purified CBL displayed natural killer (NK) activity against K562 erythroleukemic cells. Although most of these CBL were large granular lymphocytes, they lacked typical NK markers such as HNK-1 and OKM1 surface antigens. Most OKT8+, OKT3- cells were also OKT10+, Ia+ and had the receptor for peanut agglutinin. These CBL may represent a stage along the differentiation pathway leading to mature NK or T cells.

AB - It has been recently reported that cord blood lymphocytes (CBL) contain a subpopulation of OKT8+, sheep erythrocyte-rosetting negative (E-) cells not detectable in adult peripheral blood lymphocytes (a-PBL). The present studies were undertaken to characterize this subset of lymphocytes functionally and phenotypically. OKT8+ cells were purified from E-depleted CBL by negative selection on nylon-wool columns as well as by positive selection on plates coated with rabbit antibody to murine IgG (panning). The purified CBL displayed natural killer (NK) activity against K562 erythroleukemic cells. Although most of these CBL were large granular lymphocytes, they lacked typical NK markers such as HNK-1 and OKM1 surface antigens. Most OKT8+, OKT3- cells were also OKT10+, Ia+ and had the receptor for peanut agglutinin. These CBL may represent a stage along the differentiation pathway leading to mature NK or T cells.

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U2 - 10.1016/0008-8749(84)90295-8

DO - 10.1016/0008-8749(84)90295-8

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AN - SCOPUS:0021340331

SN - 0008-8749

VL - 85

SP - 252

EP - 257

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Lymphocyte subpopulations in the neonate: A subset of HNK-1^-, OKT3^-, OKT8⁺ lymphocytes displays natural killer activity

Abstract

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