TY - JOUR
T1 - Lymphocyte subpopulations in the neonate
T2 - High percentage of circulating B73.1+, HNK-1- cells
AU - Montagna, D.
AU - Ferremi, P.
AU - Alberini, C.
AU - Porta, F. A.
AU - de Amici, M.
AU - Astaldi-Ricotti, G. C.
AU - Maccario, R.
AU - Ugazio, A. G.
PY - 1986
Y1 - 1986
N2 - The reactivity of B73.1 monoclonal antibody was evaluated on unseparated and on T cell-depleted and T cell-enriched fractions of both cord blood lymphocytes (CBL) and adult peripheral blood lymphocytes (a-PBL). The reactivity of this monoclonal antibody with the E-, OKT3-, OKT8+, HNK-1- subset of CBL, previously demonstrated to have NK activity, was also studied. The data show that B73.1+ cells were present in similar percentage in CBL and a-PBL while HNK-1+ cells were very low in CBL. In addition the B73.1 monoclonal antibody reacted with about 50% of the E-, OKT3-, OKT8+, HNK-1- CBL and within this subset the majority of the B73.1+ cells were PNA-. These data support the hypothesis that part of this neonatal subpopulation, belongs to the NK cell lineage. Moreover the results obtained by double-labelling with B73.1 monoclonal antibody and PNA suggest that the E-, OKT3-, OKT8+, HNK-1- CBL include either different subsets or various differentiation stages of the same subset.
AB - The reactivity of B73.1 monoclonal antibody was evaluated on unseparated and on T cell-depleted and T cell-enriched fractions of both cord blood lymphocytes (CBL) and adult peripheral blood lymphocytes (a-PBL). The reactivity of this monoclonal antibody with the E-, OKT3-, OKT8+, HNK-1- subset of CBL, previously demonstrated to have NK activity, was also studied. The data show that B73.1+ cells were present in similar percentage in CBL and a-PBL while HNK-1+ cells were very low in CBL. In addition the B73.1 monoclonal antibody reacted with about 50% of the E-, OKT3-, OKT8+, HNK-1- CBL and within this subset the majority of the B73.1+ cells were PNA-. These data support the hypothesis that part of this neonatal subpopulation, belongs to the NK cell lineage. Moreover the results obtained by double-labelling with B73.1 monoclonal antibody and PNA suggest that the E-, OKT3-, OKT8+, HNK-1- CBL include either different subsets or various differentiation stages of the same subset.
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M3 - Article
C2 - 3750384
AN - SCOPUS:0022899193
SN - 0165-6090
VL - 8
SP - 171
EP - 176
JO - Thymus
JF - Thymus
IS - 3
ER -