TY - JOUR
T1 - Magnet Patterned Superparamagnetic Fe3O4/Au Core–Shell Nanoplasmonic Sensing Array for Label-Free High Throughput Cytokine Immunoassay
AU - Cai, Yuxin
AU - Zhu, Jingyi
AU - He, Jiacheng
AU - Yang, Wen
AU - Ma, Chao
AU - Xiong, Feng
AU - Li, Feng
AU - Chen, Weiqiang
AU - Chen, Pengyu
N1 - Funding Information:
This work was partially supported by the National Science Foundation (CBET 1701322 to W.C., CBET 1701363 to P.C.) and the National Institute of Health (R21EB025406 to W.C.). The authors would like to acknowledge Prof. Jun Cui and Dr. Xubo Liu for their help with the magnetism measurements.
Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/2/21
Y1 - 2019/2/21
N2 - Rapid and accurate immune monitoring plays a decisive role in effectively treating immune-related diseases especially at point-of-care, where an immediate decision on treatment is needed upon precise determination of the patient immune status. Derived from the emerging clinical demands, there is an urgent need for a cytokine immunoassay that offers unprecedented sensor performance with high sensitivity, throughput, and multiplexing capability, as well as short turnaround time at low system complexity, manufacturability, and scalability. In this paper, a label-free, high throughput cytokine immunoassay based on a magnet patterned Fe3O4/Au core–shell nanoparticle (FACSNP) sensing array is developed. By exploiting the unique superparamagnetic and plasmonic properties of the core–shell nanomaterials, a facile microarray patterning technique is established that allows the fabrication of a uniform, self-assembled microarray on a large surface area with remarkable tunability and scalability. The sensing performance of the FACSNP microarray is validated by real-time detection of four cytokines in complex biological samples, showing high sensitivity (≈20 pg mL−1), selectivity and throughput with excellent statistical accuracy. The developed immunoassay is successfully applied for rapid determination of the functional immunophenotype of leukemia tumor-associated macrophages, manifesting its potential clinical applications for real-time immune monitoring, early cancer detection, and therapeutic drug stratification toward personalized medicine.
AB - Rapid and accurate immune monitoring plays a decisive role in effectively treating immune-related diseases especially at point-of-care, where an immediate decision on treatment is needed upon precise determination of the patient immune status. Derived from the emerging clinical demands, there is an urgent need for a cytokine immunoassay that offers unprecedented sensor performance with high sensitivity, throughput, and multiplexing capability, as well as short turnaround time at low system complexity, manufacturability, and scalability. In this paper, a label-free, high throughput cytokine immunoassay based on a magnet patterned Fe3O4/Au core–shell nanoparticle (FACSNP) sensing array is developed. By exploiting the unique superparamagnetic and plasmonic properties of the core–shell nanomaterials, a facile microarray patterning technique is established that allows the fabrication of a uniform, self-assembled microarray on a large surface area with remarkable tunability and scalability. The sensing performance of the FACSNP microarray is validated by real-time detection of four cytokines in complex biological samples, showing high sensitivity (≈20 pg mL−1), selectivity and throughput with excellent statistical accuracy. The developed immunoassay is successfully applied for rapid determination of the functional immunophenotype of leukemia tumor-associated macrophages, manifesting its potential clinical applications for real-time immune monitoring, early cancer detection, and therapeutic drug stratification toward personalized medicine.
KW - core-shell nanomaterials
KW - label-free immunoassays
KW - microarrays
KW - nanoplasmonic
KW - superparamagnetism
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U2 - 10.1002/adhm.201801478
DO - 10.1002/adhm.201801478
M3 - Article
C2 - 30645037
AN - SCOPUS:85060142839
VL - 8
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 4
M1 - 1801478
ER -