Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus

Ji Young Min; Celia Santos; Adam Fitch; Alan Twaddle; Yoshiko Toyoda; Jay V. DePasse; Elodie Ghedin; Kanta Subbarao

doi:10.1128/JVI.01016-13

Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus

Ji Young Min, Celia Santos, Adam Fitch, Alan Twaddle, Yoshiko Toyoda, Jay V. DePasse, Elodie Ghedin, Kanta Subbarao

Research output: Contribution to journal › Article › peer-review

Abstract

The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Original language	English (US)
Pages (from-to)	10884-10888
Number of pages	5
Journal	Journal of virology
Volume	87
Issue number	19
DOIs	https://doi.org/10.1128/JVI.01016-13
State	Published - 2013

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.01016-13

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abstract = "The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.",

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AU - Santos, Celia

AU - Fitch, Adam

AU - Twaddle, Alan

AU - Toyoda, Yoshiko

AU - DePasse, Jay V.

AU - Ghedin, Elodie

AU - Subbarao, Kanta

PY - 2013

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AB - The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

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JO - Journal of virology

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Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus

Abstract

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