Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection

Shuang Wang, Marina Ruiz de Galarreta, Kirsten C. Sadler, Amaia Lujambio

Research output: Contribution to journalArticlepeer-review

Abstract

Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements into hepatocytes. Transfected hepatocytes can then be traced with a GFP reporter enabling the use of immunohistochemistry and FACS sorting to examine the changes in hepatocyte gene expression and proliferation during liver regeneration induced by 2/3 partial hepatectomy (PH). For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).

Original languageEnglish (US)
Article number100440
JournalSTAR Protocols
Volume2
Issue number2
DOIs
StatePublished - Jun 18 2021

Keywords

  • Cell Biology
  • Flow Cytometry/Mass Cytometry
  • Metabolism
  • Microscopy
  • Model Organisms
  • Molecular Biology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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