Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors

Romain Durand De Cuttoli; Sarah Mondoloni; Fabio Marti; Damien Lemoine; Claire Nguyen; Jérémie Naudé; Thibaut D’izarny-Gargas; Stéphanie Pons; Uwe Maskos; Dirk Trauner; Richard H. Kramer; Philippe Faure; Alexandre Mourot

doi:10.7554/eLife.37487

Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors

Romain Durand De Cuttoli, Sarah Mondoloni, Fabio Marti, Damien Lemoine, Claire Nguyen, Jérémie Naudé, Thibaut D’izarny-Gargas, Stéphanie Pons, Uwe Maskos, Dirk Trauner, Richard H. Kramer, Philippe Faure, Alexandre Mourot

Chemistry

Research output: Contribution to journal › Article

Abstract

Dopamine (DA) neurons of the ventral tegmental area (VTA) integrate cholinergic inputs to regulate key functions such as motivation and goal-directed behaviors. Yet the temporal dynamic range and mechanism of action of acetylcholine (ACh) on the modulation of VTA circuits and reward-related behaviors are not known. Here, we used a chemical-genetic approach for rapid and precise optical manipulation of nicotinic neurotransmission in VTA neurons in living mice. We provide direct evidence that the ACh tone fine-tunes the firing properties of VTA DA neurons through β2-containing (β2*) nicotinic ACh receptors (nAChRs). Furthermore, locally photo-antagonizing these receptors in the VTA was sufficient to reversibly switch nicotine reinforcement on and off. By enabling control of nicotinic transmission in targeted brain circuits, this technology will help unravel the various physiological functions of nAChRs and may assist in the design of novel therapies relevant to neuropsychiatric disorders.

Original language	English (US)
Article number	e37487
Journal	eLife
Volume	7
DOIs	https://doi.org/10.7554/eLife.37487
State	Published - Sep 2018

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ASJC Scopus subject areas

Neuroscience(all)
Immunology and Microbiology(all)
Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cuttoli, R. D. D., Mondoloni, S., Marti, F., Lemoine, D., Nguyen, C., Naudé, J., D’izarny-Gargas, T., Pons, S., Maskos, U., Trauner, D., Kramer, R. H., Faure, P., & Mourot, A. (2018). Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. eLife, 7, [e37487]. https://doi.org/10.7554/eLife.37487

Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. / Cuttoli, Romain Durand De; Mondoloni, Sarah; Marti, Fabio; Lemoine, Damien; Nguyen, Claire; Naudé, Jérémie; D’izarny-Gargas, Thibaut; Pons, Stéphanie; Maskos, Uwe; Trauner, Dirk; Kramer, Richard H.; Faure, Philippe; Mourot, Alexandre.

In: eLife, Vol. 7, e37487, 09.2018.

Research output: Contribution to journal › Article

Cuttoli, Romain Durand De ; Mondoloni, Sarah ; Marti, Fabio ; Lemoine, Damien ; Nguyen, Claire ; Naudé, Jérémie ; D’izarny-Gargas, Thibaut ; Pons, Stéphanie ; Maskos, Uwe ; Trauner, Dirk ; Kramer, Richard H. ; Faure, Philippe ; Mourot, Alexandre. / Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. In: eLife. 2018 ; Vol. 7.

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abstract = "Dopamine (DA) neurons of the ventral tegmental area (VTA) integrate cholinergic inputs to regulate key functions such as motivation and goal-directed behaviors. Yet the temporal dynamic range and mechanism of action of acetylcholine (ACh) on the modulation of VTA circuits and reward-related behaviors are not known. Here, we used a chemical-genetic approach for rapid and precise optical manipulation of nicotinic neurotransmission in VTA neurons in living mice. We provide direct evidence that the ACh tone fine-tunes the firing properties of VTA DA neurons through β2-containing (β2*) nicotinic ACh receptors (nAChRs). Furthermore, locally photo-antagonizing these receptors in the VTA was sufficient to reversibly switch nicotine reinforcement on and off. By enabling control of nicotinic transmission in targeted brain circuits, this technology will help unravel the various physiological functions of nAChRs and may assist in the design of novel therapies relevant to neuropsychiatric disorders.",

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Access to Document

10.7554/eLife.37487