TY - JOUR
T1 - Mapping a cave fish genome
T2 - Polygenic systems and regressive evolution
AU - Borowsky, Richard
AU - Wilkens, H.
N1 - Funding Information:
From the Cave Biology Research Group, Department of Biology, 1009 Main, New York University, Washington Square, NY 10003 (Borowsky) and Zoology Institute and Zoology Museum of the University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany (Wilkens). We thank M. McClelland and J. Welsh for support and advice during the early stages of this study. Technical help was provided by B. Andiak, J. Khlevner, and J. Starobinets. This study was partly funded by a Research Challenge Fund grant from New York University (to R.B.). Address correspondence to Richard Borowsky at the address above or e-mail: [email protected].
PY - 2002
Y1 - 2002
N2 - We used random amplified polymorphic DNA (RAPD) fingerprinting to generate anonymous DNA markers in the fish Astyanax mexicanus, a species with both surface and cave populations. Surface individuals are eyed and pigmented; troglobitic forms are blind and depigmented. We hybridized surface fish and Pachon population cave fish and produced a RAPD genomic map 1064 cM in length (about half the total length of the genome) that was used to screen for quantitative trait loci (QTL) for troglomorphic traits. Three QTL for reduced eye size, two for decreased numbers of melanophores, two for condition factor, and the locus for the unifactorial trait, albinism, were mapped. These factors account for an average of 46% of the variance in these traits in the backcross. The results are the first direct demonstration that troglomorphic changes in this population are multifactorial. Two closely linked pairs of QTL were found. Each consisted of a regressive and a constructive trait QTL. These close linkages are unlikely to have occurred by chance (P < .05 for each) and suggest that troglomorphic evolution might be facilitated by pleiotropy or by genetic hitchhiking.
AB - We used random amplified polymorphic DNA (RAPD) fingerprinting to generate anonymous DNA markers in the fish Astyanax mexicanus, a species with both surface and cave populations. Surface individuals are eyed and pigmented; troglobitic forms are blind and depigmented. We hybridized surface fish and Pachon population cave fish and produced a RAPD genomic map 1064 cM in length (about half the total length of the genome) that was used to screen for quantitative trait loci (QTL) for troglomorphic traits. Three QTL for reduced eye size, two for decreased numbers of melanophores, two for condition factor, and the locus for the unifactorial trait, albinism, were mapped. These factors account for an average of 46% of the variance in these traits in the backcross. The results are the first direct demonstration that troglomorphic changes in this population are multifactorial. Two closely linked pairs of QTL were found. Each consisted of a regressive and a constructive trait QTL. These close linkages are unlikely to have occurred by chance (P < .05 for each) and suggest that troglomorphic evolution might be facilitated by pleiotropy or by genetic hitchhiking.
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U2 - 10.1093/jhered/93.1.19
DO - 10.1093/jhered/93.1.19
M3 - Article
C2 - 12011170
AN - SCOPUS:0036257138
SN - 0022-1503
VL - 93
SP - 19
EP - 21
JO - Journal of Heredity
JF - Journal of Heredity
IS - 1
ER -