Mass-based readout for agglutination assays

Rumi Chunara; Michel Godin; Scott M. Knudsen; Scott R. Manalis

doi:10.1063/1.2806197

Mass-based readout for agglutination assays

Rumi Chunara, Michel Godin, Scott M. Knudsen, Scott R. Manalis

Global Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

Original language	English (US)
Article number	193902
Journal	Applied Physics Letters
Volume	91
Issue number	19
DOIs	https://doi.org/10.1063/1.2806197
State	Published - 2007

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.2806197

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title = "Mass-based readout for agglutination assays",

abstract = "We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.",

author = "Rumi Chunara and Michel Godin and Knudsen, {Scott M.} and Manalis, {Scott R.}",

note = "Funding Information: We thank Geoffrey von Maltzahn, Amit Agrawal, and Ken Babcock for valuable discussions and acknowledge financial support from the National Institutes of Health (NIH) Cell Decision Process Center Grant and the Institute for Collaborative Biotechnologies from the U.S. Army Research Office. M.G. acknowledges support from the Natural Sciences and Engineering Research Council of Canada (NSERC) through a postdoctoral fellowship.",

year = "2007",

doi = "10.1063/1.2806197",

language = "English (US)",

volume = "91",

journal = "Applied Physics Letters",

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AU - Chunara, Rumi

AU - Godin, Michel

AU - Knudsen, Scott M.

AU - Manalis, Scott R.

N1 - Funding Information: We thank Geoffrey von Maltzahn, Amit Agrawal, and Ken Babcock for valuable discussions and acknowledge financial support from the National Institutes of Health (NIH) Cell Decision Process Center Grant and the Institute for Collaborative Biotechnologies from the U.S. Army Research Office. M.G. acknowledges support from the Natural Sciences and Engineering Research Council of Canada (NSERC) through a postdoctoral fellowship.

PY - 2007

Y1 - 2007

N2 - We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

AB - We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

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Mass-based readout for agglutination assays

Abstract

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